Pattern of F-18 FDG Uptake in Colon Cancer after Bacterial Cancer Therapy Using Engineered Salmonella Typhimurium: A Preliminary In Vivo Study

IF 2.8 4区 医学 Q2 Medicine
A. Chong, Dinh-Huy Nguyen, H. Kim, June-Key Chung, J. Min
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引用次数: 2

Abstract

Purpose Bacterial cancer therapy (BCT) research using engineered Salmonella typhimurium has increased in recent years. 2-Deoxy-2[18F] fluoro-D-glucose positron emission tomography (FDG PET) is widely used in cancer patients to detect cancer, monitor treatment responses, and predict prognoses. The aim of this pilot study was to investigate FDG uptake patterns in a mouse tumor model after BCT. Procedures. BCT was performed via the intravenous injection of attenuated S. typhimurium (SLΔppGpp/lux) into female mice bearing a tumor (derived from CT26 murine colon cancer cells) in the right thigh. 18F-FDG PET images acquired before BCT and at different time points after BCT. In vivo bioluminescence imaging confirmed bacterial presence in the tumor. The tumor volume, standardized uptake value (SUV) of FDG (SUVmax and SUVmean), early SUV reduction%, and normalized tumor volume change were analyzed. Results Early after BCT (1 or 2 days post-injection (dpi)), FDG tumor uptake decreased in 10 out of 11 mice and then increased at later stages. FDG uptake before BCT was correlated with normalized tumor volume change after BCT. Early FDG reduction% after BCT was correlated with normalized volume change after BCT. Conclusions Early after BCT, FDG tumor uptake decreased and then increased at later stages. The higher the FDG tumor uptake before BCT, the better the BCT response. FDG uptake patterns were related to tumor volume change after BCT. Therefore, FDG uptake was a good candidate for evaluating BCT.
基因型鼠伤寒沙门氏菌细菌癌症治疗后结肠癌癌症F-18 FDG摄取模式的体内初步研究
目的近年来,利用鼠伤寒沙门氏菌进行细菌癌症治疗的研究有所增加。2-脱氧-2[18F]氟-D-葡萄糖正电子发射断层扫描(FDG PET)广泛用于癌症患者检测癌症、监测治疗反应和预测预后。这项初步研究的目的是研究BCT后小鼠肿瘤模型中FDG的摄取模式。程序。通过将减毒鼠伤寒杆菌(SLΔppGpp/lux)静脉注射到右大腿带有肿瘤(来源于CT26小鼠癌症结肠癌细胞)的雌性小鼠中进行BCT。在BCT前和BCT后不同时间点采集的18F-FDG PET图像。体内生物发光成像证实肿瘤中存在细菌。分析肿瘤体积、FDG的标准化摄取值(SUV)(SUVmax和SUVmean)、早期SUV减少%和标准化肿瘤体积变化。结果BCT后早期(注射后1或2天(dpi)),11只小鼠中有10只FDG肿瘤摄取量下降,然后在后期增加。BCT前的FDG摄取与BCT后的正常肿瘤体积变化相关。BCT后早期FDG减少%与BCT后的归一化体积变化相关。结论BCT术后早期FDG肿瘤摄取量先下降后上升。BCT前FDG肿瘤摄取量越高,BCT反应越好。FDG摄取模式与BCT后肿瘤体积变化有关。因此,FDG摄取是评估BCT的一个很好的候选者。
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来源期刊
Molecular Imaging
Molecular Imaging 生物-核医学
CiteScore
4.50
自引率
3.60%
发文量
21
审稿时长
>12 weeks
期刊介绍: Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.
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