Delonix Regia Bark Alleviated Inflammation by Stimulating Defense Responses in Rats

IF 1.2 Q4 PHARMACOLOGY & PHARMACY

Journal of Herbs, Spices and Medicinal Plants Pub Date : 2021-10-02 DOI:10.1080/10496475.2021.1943591

A. Bhaskar, Shailasree Sekhar

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引用次数: 0

Abstract

ABSTRACT The methanol extract of Delonix regia bark (DMeOH) was investigated for its anti-inflammatory property in kappa-carrageenan (Carr)-induced hind paw edema of rats. DMeOH was orally administered and evaluated as a measure of edema, tissue destruction, and neutrophil migration to the inflammation site. The pro-inflammatory mediators, p-p65 subunit of NFκB, interleukin (IL)-1β, cycloxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) were analyzed. Catalase (CAT) and superoxide dismutase (SOD) enzyme activity assays and malondialdehye (MDA) were quantified. The LC-MS fingerprint of the extract identified 10 polyphenols, of which five were structurally characterized based on their [M-H]− peak, UV-vis absorption compared to published data. Anti-inflammatory studies revealed that pre-treatment with DMeOH (0.5 g kg−1) prior to Carr-injection inhibited paw edema abating tissue destruction with decreased neutrophil infiltration. Carr-injection resulted in increased NO(•-) production, iNOS transcript and p-p65 subunit of NF-κB protein. DMeOH feed inhibited NO(•-) production and iNOS transcript levels. A decreased expression of NF-κB p-p65 subunit, COX-2 and IL-1β at 0.5 g kg−1 b.w oral feed suggest that DMeOH exerted its anti-inflammatory property by inhibiting NFκB activation. An increase in the antioxidant enzyme activities of CAT (78%) and SOD (49%) with reduced MDA levels was recorded.

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龙葵皮通过刺激大鼠防御反应减轻炎症反应

摘要:本文研究了帝龙树皮甲醇提取物(DMeOH)对卡帕-卡拉胶(Carr)诱导的大鼠后足水肿的抗炎作用。口服DMeOH，并评估其水肿、组织破坏和中性粒细胞向炎症部位迁移的程度。分析促炎介质、NFκB的p-p65亚基、白细胞介素(IL)-1β、环氧化酶(COX)-2和诱导型一氧化氮合酶(iNOS)。测定过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。提取液的LC-MS指纹图谱鉴定出10种多酚，其中5种多酚的[M-H]−峰和紫外-可见吸收光谱与已发表的数据进行了结构表征。抗炎研究显示，在carr注射前用DMeOH (0.5 g kg - 1)预处理可抑制足跖水肿，减轻组织破坏，减少中性粒细胞浸润。carr注射导致NO(•-)生成、iNOS转录和NF-κ b蛋白p-p65亚基升高。DMeOH饲料抑制NO(•-)生成和iNOS转录水平。0.5 g kg -1 b.w口服饲料可降低NF-κB p-p65亚基、COX-2和IL-1β的表达，表明DMeOH通过抑制NF-κB活化发挥其抗炎作用。随着MDA水平的降低，CAT(78%)和SOD(49%)的抗氧化酶活性升高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Herbs, Spices and Medicinal Plants Medicine-Complementary and Alternative Medicine

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.