Evidence of ancestral nocturnality, locomotor clock regression, and cave zone-adjusted sleep duration modes in a cave beetle

IF 1.5 3区 生物学 Q2 ZOOLOGY
Sonya Royzenblat, Jasmina Kulacic, M. Friedrich
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引用次数: 0

Abstract

The small carrion beetle Ptomaphagus hirtus is an abundant inhabitant of the exceptionally biodiverse Mammoth Cave system. Previous studies revealed negative phototaxis and the expression of biological clock genes in this microphthalmic cave beetle. Here we present results from probing P. hirtus for the entrainment of locomotor rhythms using the TriKinetics activity monitor setup. Although curtailed by low adjustment frequency of animals to the test environment, the data obtained from successfully monitoring two animals in constant darkness (DD) and six animals exposed to 12 hour light-dark cycles (LD) revealed a strong effect of light on locomotor activity in P. hirtus. In LD, activity was prevalent during the artificial night phases while close to absent during the presumptive day phases, suggesting conserved nocturnality. Upon transitioning LD animals to constant darkness, none displayed detectable evidence of free-running activity rhythms, suggesting complete regression of the central circadian clock. Equally notable, overall locomotor activity of the two DD-monitored animals was about three-fold lower compared to LD animals due to longer rest durations in the former. We, therefore, propose the existence of cave zone-specific energy expenditure modes that are mediated through light schedule responsive modification of sleep duration in P. hirtus.
Evidence祖先的夜间活动,运动时钟回归,以及洞穴甲虫的洞穴区域调节睡眠持续时间模式
小型腐肉甲虫Ptomaphagus hirtus是生物多样性极高的猛犸洞穴系统中的丰富居民。先前的研究揭示了这种小眼洞甲虫的负趋光性和生物钟基因的表达。在这里,我们展示了使用TriKinetics活动监测器设置探测P.hirtus是否携带运动节律的结果。尽管由于动物对测试环境的调整频率较低而受到限制,但通过成功监测持续黑暗(DD)中的两只动物和暴露于12小时明暗循环(LD)中的六只动物获得的数据显示,光对P.hirtus的运动活动有很强的影响。在LD中,活动在人工夜间阶段普遍存在,而在假定的白天阶段几乎不存在,这表明保守的夜间性。在LD动物过渡到持续的黑暗中时,没有一只动物表现出可检测到的自由活动节律的证据,这表明中枢昼夜节律完全退化。同样值得注意的是,由于前者的休息时间更长,两只DD监测动物的整体运动活性比LD动物低约三倍。因此,我们提出了洞穴区特定能量消耗模式的存在,这些模式是通过光照时间表反应性地改变P.hirtus的睡眠时间来介导的。
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来源期刊
CiteScore
2.50
自引率
18.20%
发文量
28
审稿时长
6 weeks
期刊介绍: Subterranean Biology is an international journal published by Pensoft on behalf of the International Society for Subterranean Biology [SIBIOS-ISSB] for the publication of original and significant contributions on all aspects of subterranean life. The Journal will consider original scientific papers dealing with any aspect of subterranean ecosystems and their components focusing on biology, ecology, evolution and conservation. Reviews and Commentaries: The journal welcomes review articles on aspects of subterranean biology. They should be submitted in the same way as research articles but use a simple layout with no more than three levels of headings. Commentaries on previous matter published in the journal may be also considered. Both reviews and commentaries will be reviewed. Book Reviews: Reviews of books, symposia volumes and conferences pertinent to the journal are welcome. The journal publishes electronic versions of the articles when these are ready to publish, without delays that might be caused by completion of an issue. These electronic versions are not "pre-prints" but final and immutable (Version of Record), hence available for the purposes of biological nomenclature. The date indicated on the electronic version is to be considered the actual publication date.
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