Anti-Oxidative Effect of Aqueous Garlic Extract (AGE) on Androgen Induced Changes in Ovaries of Prepubertal Female Rats

Abstract

Objectives: It has been documented that the administration of exogenous androgens to immature female rats produces polycystic ovaries. There is a substantial reduction of antioxidants in this condition, with an elevated risk of oxidative stress. The current research is intended to evaluate these effects and to assess the protection provided by aqueous garlic extract (AGE). Methods: An experimental study conducted at University of Health Sciences, Lahore. The data was collected over a period of one month. Fifty female prepubertal rats, 21 days of age, were divided into five groups, A, B, C, D and E. Group A served as control. Group B received testosterone propionate (TP) subcutaneous for 14 days and served as disease control. Group C received testosterone propionate (TP) subcutaneous for 14 days and concomitantly Aqueous garlic extract (AGE). Group D receive testosterone propionate (TP) subcutaneous for 14 days and Aqueous garlic extract (AGE) from day 14-21. Group E received testosterone propionate (TP) subcutaneous for 14 days with no intervention till day 21. Blood samples of 50 female rats were drawn by doing cardiac puncture and clear serum was collected by centrifugation. This serum was used to assess the Catalase enzyme by using specific commercial kits. Results: The concentration and activity of catalase enzyme in the female rats with polycystic ovaries showed significant decrease as compare to the healthy controls. The involvement of antioxidants to manage the polycystic ovaries may be helpful as secondary therapy to prevent oxidative damage. Conclusion: The results showed that AGE with its antioxidative properties not only prevents the damage caused by oxidative stress, it also increased the level of serum catalase that helps to create a balance between beneficial oxidant generation and damaging oxidative stress. Key words: Androgens, immature female rats, ovaries, antioxidants, oxidative stress, aqueous garlic extract (AGE), Catalase. How to cite: Bashir.Y., Habib N. Ashraf .S. Anti-Oxidative Effect of Aqueous Garlic Extract (AGE) on Androgen Induced Changes in Ovaries of Prepubertal Female Rats. Esculapio. 2021.17(01):55-59