Genetic improvement of faba bean (Vicia faba L.) genotypes selected for resistance to chocolate spot disease

Q4 Agricultural and Biological Sciences

Agronomia Colombiana Pub Date : 2022-05-01 DOI:10.15446/agron.colomb.v40n2.102128

Hany Elsayed Heiba, E. Mahgoub, A. Mahmoud, M. Ibrahim, E. Mahdy

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引用次数: 3

Abstract

Inter-varietal hybridization is a powerful tool for genetic improvement and production of new genotypes for a trait of interest. Four parents of faba beans (Vicia faba L.) were hybridized using agromorphological and molecular characterization to obtain genotypes resistant to the chocolate spot disease. The study was done at the Nubaria Research Station, Giza, Egypt. Eight traits including resistance to chocolate spot, days to flowering, plant height (cm), number of branches/plant, number of pods/plant, number of seeds/plant, 100-seed weight (g), and seed yield/plant were estimated during the three growth seasons of 2016/2017, 2017/2018, and 2018/2019. Genetic parameters revealed by RAPD and ISSR markers assessed the genetic variation of genotypes with their generations. Crosses 1 (P1 “Nubaria-1” x P2 “Sakha-1”), 2 (P1 “Nubaria-1” x P3 “T.W”), and 3 (P1 “Nubaria-1” x P4 “Camolina”) revealed high resistance to disease with high yield. Markers patterned specific loci of resistant parents at a length of 360, 470, 450, 660, and 140 bp in RAPD and 1100, 810, 650, 700, 480 bp in ISSR. Inter-varietal hybridization between the resistant and susceptible genotypes is considered one of the most promising methods to obtain germplasm with resistance and high yield.

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蚕豆抗巧克力斑点病基因型的遗传改良

品种间杂交是遗传改良和为感兴趣的性状产生新基因型的有力工具。利用农业形态学和分子生物学特性对蚕豆（Vicia faba L.）的四个亲本进行杂交，获得对巧克力斑点病具有抗性的基因型。这项研究是在埃及吉萨的努比亚研究站进行的。在2016/2017、2017/2018和2018/2019三个生长季节，估计了八个性状，包括对巧克力斑点的抗性、开花天数、株高（cm）、分枝/株数、荚数/株数/种子数、百粒重（g）和种子产量/株。RAPD和ISSR标记揭示的遗传参数评估了基因型随世代的遗传变异。杂交种1（P1“Nubaria-1”x P2“Sakha-1”）、2（P1“Nubaria-1”x P3“T.W”）和3（P1“努比亚-1”x P4“Camolina”）表现出高抗病性和高产。标记在RAPD中以360470450660和140bp的长度和在ISSR中以1100810650700480bp的长度图案化抗性亲本的特定基因座。抗性和感病基因型的品种间杂交被认为是获得抗性和高产种质的最有前途的方法之一。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Agronomia Colombiana Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

25 weeks

期刊介绍： Agronomia Colombiana journal it is intended to transfer research results in different areas of tropical agronomy. Original unpublished papers are therefore accepted in the following areas: physiology, crop nutrition and fertilization, genetics and plant breeding, entomology, phytopathology, integrated crop protection, agro ecology, weed science, environmental management, geomatics, biometry, soils, water and irrigation, agroclimatology and climate change, post-harvest and agricultural industrialization, food technology, rural and agricultural entrepreneurial development, agrarian economy, and agricultural marketing (Published: Quarterly).