The Study of mRNA Expression Profiles of Main Cell Function Regulator Genes in Unchanged Colon Mucosa from Healthy Donors

IF 0.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Pub Date : 2022-02-14 DOI:10.1134/S1990750822010115

M. V. Zakharenko, V. K. Bozhenko, Ya. Yu. Kiseleva, E. L. Dzhikiya, U. S. Stanoevich, T. M. Kulinich, N. V. Melnikova, A. L. Senchukova, A. B. Urakova, I. B. Grunin, S. V. Goncharov, O. P. Bliznyukov, V. A. Solodkiy

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Abstract

A comparative analysis of molecular genetic phenotypes of mucous membrane cells has been carried out in five anatomical regions of the large intestine in a group of healthy donors. The mRNA expression profiles of 62 genes involved in the regulation of vital cellular function were investigated in 181 biopsy samples of morphologically unchanged colon mucosa, obtained from the colon (ascending, transverse, descending, sigmoid) and rectum sections during prophylactic colonoscopy of 58 donors without large intestine pathology. The mRNA levels for 62 genes involved in the regulation of apoptosis, proliferation, transcription, differentiation, cell-cell adhesion, and immune response were assessed by RT-PCR. Statistically significant differences were found for the molecular phenotypes of five sections of the large intestine. The results of the study can serve as a basis for creating a reference database (values of expression profiles), developing methods of differential diagnostics and screening of various pathologies of the large intestine.

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健康供体未改变结肠黏膜主要细胞功能调控基因mRNA表达谱的研究

在一组健康供体大肠的五个解剖区域中，对粘膜细胞的分子遗传表型进行了比较分析。在58例无大肠病理的供者的预防性结肠镜检查中，从结肠(升结肠、横结肠、降结肠、s型结肠)和直肠部分获得181个形态学不变的结肠粘膜活检样本，研究了62个参与重要细胞功能调控的基因的mRNA表达谱。采用RT-PCR技术检测了参与细胞凋亡、增殖、转录、分化、细胞-细胞粘附和免疫应答调控的62个基因的mRNA水平。在大肠的五个部分的分子表型上发现了统计学上显著的差异。本研究结果可作为建立参考数据库(表达谱值)、开发鉴别诊断方法和筛选大肠各种病理的基础。

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来源期刊

Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry covers all major aspects of biomedical chemistry and related areas, including proteomics and molecular biology of (patho)physiological processes, biochemistry, neurochemistry, immunochemistry and clinical chemistry, bioinformatics, gene therapy, drug design and delivery, biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine. The journal also publishes review articles. All issues of the journal usually contain solicited reviews.