{"title":"Lipid Membranes Electroporation Cannot Be Described by the Constant Line Tension Model of the Pore Edge","authors":"P. K. Gifer, O. V. Batishchev","doi":"10.1134/S1990747823040050","DOIUrl":null,"url":null,"abstract":"<p>We have studied the process of electroporation of bilayer lipid membranes (BLMs) made of dioleoylphosphatidylcholine (DOPC). We obtained experimental data on the average lifetime of the membrane as a function of applied transmembrane voltage in the range of 200–375 mV. The analysis of the obtained data showed that the dependence is nonmonotonic and cannot be described in terms of the classical theory of electroporation. These results are consistent with modern models of the process of formation of through conductive pores in a membrane. The above models imply a complex pore energy profile and its dependence on a membrane tension and an external electric field. Thus, we have shown that the classical theory of electroporation does not satisfy the experimentally observed dependencies of the average membrane lifetime on the applied potential difference and requires further refinement.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 3","pages":"195 - 199"},"PeriodicalIF":1.1000,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","FirstCategoryId":"2","ListUrlMain":"https://link.springer.com/article/10.1134/S1990747823040050","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
We have studied the process of electroporation of bilayer lipid membranes (BLMs) made of dioleoylphosphatidylcholine (DOPC). We obtained experimental data on the average lifetime of the membrane as a function of applied transmembrane voltage in the range of 200–375 mV. The analysis of the obtained data showed that the dependence is nonmonotonic and cannot be described in terms of the classical theory of electroporation. These results are consistent with modern models of the process of formation of through conductive pores in a membrane. The above models imply a complex pore energy profile and its dependence on a membrane tension and an external electric field. Thus, we have shown that the classical theory of electroporation does not satisfy the experimentally observed dependencies of the average membrane lifetime on the applied potential difference and requires further refinement.
期刊介绍：
Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.
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