The Role of Phosphocreatine in the Perconditioning and Postconditioning of Isolated Rat Heart

Q3 Medicine

Serbian Journal of Experimental and Clinical Research Pub Date : 2022-06-01 DOI:10.2478/sjecr-2022-0021

Tanja Jesic Petrovic, L. Gadzieva, Stefani S Bolevich, I. A. Omarov, Marija Kartashova, S. Bolevich

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Abstract

Abstract The present study strives to assess the cardioprotective role of phosphocreatine as an agent for postconditioning and perconditioning of isolated rat heart. Rat hearts (n=30) were perfused with a Langendorff apparatus and randomly assigned to three groups subjected to 20 minutes of global ischemia and 30 minutes of reperfusion: control group (untreated rat hearts), postconditioning group (hearts treated with 0.2 mmol/l of phosphocreatine during the first 5 minutes of reperfusion), and perconditioning group (hearts treated with 0.2 mmol/l of phosphocreatine during the first 5 minutes of ischemia). During the experimental protocol, cardiodynamic parameters were evaluated, while oxidative stress parameters such as superoxide anion radical, hydrogen peroxide, nitrites and index of lipid peroxidation were determined in coronary venous effluent. Postconditioning and perconditioning with phosphocreatine improved contractile function, heart rate and coronary flow, while the examined oxidative stress parameters in coronary venous effluent were significantly reduced in groups of treated rat hearts. The results of this study indicate that phosphocreatine has the potential as a therapeutic agent for perconditioning and postconditioning the heart in ischemia reperfusion injury.

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磷酸肌酸在离体大鼠心脏渗透和后处理中的作用

摘要本研究旨在评估磷酸肌酸作为大鼠离体心脏后处理和过处理剂的心脏保护作用。用Langendorff装置灌注大鼠心脏（n＝30）并将其随机分配到经历20分钟全缺血和30分钟再灌注的三组：对照组（未处理的大鼠心脏）、后处理组（在再灌注的前5分钟用0.2mmol/l磷酸肌酸处理的心脏）、，和perconditioning组（在缺血的前5分钟用0.2mmol/l磷酸肌酸处理的心脏）。在实验方案中，评估了心脏动力学参数，同时测定了冠状静脉流出液中的氧化应激参数，如超氧阴离子自由基、过氧化氢、亚硝酸盐和脂质过氧化指数。磷酸肌酸后处理和过处理改善了收缩功能、心率和冠状动脉流量，而在处理的大鼠心脏组中，冠状静脉流出液中检测的氧化应激参数显著降低。本研究结果表明，磷酸肌酸具有作为缺血再灌注损伤中心脏感知和后感知的治疗剂的潜力。

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来源期刊

Serbian Journal of Experimental and Clinical Research Medicine-Medicine (all)

自引率

0.00%

发文量

审稿时长

6 weeks

期刊介绍： Serbian Journal of Experimental and Clinical Research is a peer-reviewed, general biomedical journal. It publishes original basic and clinical research, clinical practice articles, critical reviews, case reports, evaluations of scientific methods, works dealing with ethical and social aspects of biomedicine as well as letters to the editor, reports of association activities, book reviews, news in biomedicine, and any other article and information concerned with practice and research in biomedicine, written in the English.