Effect of low-density lipoproteins and trehalose on the quality of cryopreserved bovine semen

IF 0.2 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Revista Colombiana De Ciencias Pecuarias Pub Date : 2020-08-10 DOI:10.17533/udea.rccp.v34n3a03

Elizabeth Varela-Giraldo, Alexandra Úsuga-Suárez, Juan E Uque-Cortés, Jorge Gómez-Oquendo, Giovanni Restrepo-Betancur

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引用次数: 0

Abstract

Background: In artificial insemination, chicken egg yolk is added to bovine semen to protect it during the cryopreservation process, although it contains substances that can affect the microbiological quality and metabolism of sperm. Objective: To evaluate post-thaw quality of bovine cryopreserved semen added with centrifuged and non-centrifuged egg yolk, low-density lipoproteins (LDL), and trehalose (T). Methods: Ten ejaculates from five bulls were cryopreserved under the treatments T1: pure egg yolk (PEY) at 20% v/v, T2: centrifuged egg yolk (CEY) at 20% v/v, T3: LDL at 8% v/v, T4: T at 100 mM, and T5: T at 100 mM plus LDL at 8% v/v (TLDL). Spermatic motility and kinetics, functional membrane integrity (FMI), structural membrane integrity (SMI), sperm vitality (SV) and abnormal morphology (AM) were assessed using the Sperm Class Analyzer (SCA®) system, hypoosmotic test (HOST), SYBR/PI probes, and eosin–nigrosin staining, respectively. A completely randomized design was used. Normal distribution of the variables was validated through the Kolmogórov– Smirnov test. A generalized linear model was used to determine sources of variation. Means were compared using the Tukey test. Results: Inclusion of CEY or LDL had a similar effect on sperm protection, and were superior for motility, kinetics and membrane integrity compared to the other treatments (p<0.05). CEY was superior for progressive motility (p<0.05). The cryoprotective action of LDL was similar to TLDL for motility and kinetics, SMI, SV, and AM (p<0.05). Inclusion of PEY and T resulted in the lowest semen quality (p<0.05). The use of T resulted in a reduction in FMI and SMI (p<0.05). No differences in AM between treatments were found (p>0.05). Conclusions: Egg yolk can be replaced by centrifuged egg yolk or low-density lipoproteins in the freezing extender used for bovine semen used in artificial insemination.

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低密度脂蛋白和海藻糖对冷冻保存牛精液质量的影响

背景：在人工授精中，将蛋黄添加到牛精液中，以在冷冻保存过程中对其进行保护，尽管蛋黄中含有会影响精子微生物质量和代谢的物质。目的：评价添加了离心和非离心蛋黄、低密度脂蛋白（LDL）和海藻糖（T）的牛冷冻精液解冻后的质量。方法：将5头公牛的10支精液冷冻保存在处理T1：20%v/v的纯蛋黄（PEY），T2：20%v/v的离心蛋黄（CEY），T3:8%v/v的低密度脂蛋白，T4:T在100mM，T5:T在100%v/v的高密度脂蛋白（TLDL）下。分别使用精子类别分析仪（SCA®）系统、低渗透测试（HOST）、SYBR/PI探针和曙红-黑色素染色评估精子运动和动力学、功能膜完整性（FMI）、结构膜完整性、精子活力和异常形态。采用完全随机设计。通过Kolmogórov–Smirnov检验验证了变量的正态分布。使用广义线性模型来确定变异源。使用Tukey检验比较平均值。结果：与其他处理相比，CEY或LDL对精子的保护作用相似，在运动性、动力学和膜完整性方面均优于其他处理（p0.05）。

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来源期刊

Revista Colombiana De Ciencias Pecuarias AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

0.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The editors of Revista Colombiana de Ciencias Pecuarias (RCCP) welcome the submission of original manuscripts on experimental and clinical studies associated with the broad areas of animal sciences and veterinary medicine as they interface with biochemistry, molecular biology, physiology, pharmacology, toxicology, pathology, microbiology, parasitology, immunology and epidemiology. The scope of the journal includes studies of basic and applied research in animal management and production, feeding and nutrition, reproduction, breeding, genetics, animal welfare and behavior; as well as animal production focussed from biotechnology, soil science, agrostology, silvopastoral systems, livestock economics and the environment. The criteria for acceptance of papers submitted for publication are originality, quality and clarity of the content. Each contribution must be based on original, unpublished research that has not been simultaneously submitted to other journals. All papers will be peer reviewed. All authors bear responsibility for ensuring the integrity and quality of their reported research. It is the author''s responsibility to secure permission to use figures or tables that have been published elsewhere. Contributions may be classified as original research, review, rapid communication, clinical case studies or methodological articles, as well as news/commentaries or letters to the editor. Most review articles are invited by the editor. Authors interested in submitting a review article should contact the corresponding editor. Rapid publication of original manuscripts is a goal of the journal. Manuscripts must be written in English. Each manuscript is considered for publication with the understanding that it has not been simultaneously submitted to any other journal. Upon acceptance for publication, papers are subject to editorial review and revision.