Variability and Structure of the Repetitive Region of the Major Royal Jelly Protein Gene mrjp3 in Honeybee Apis mellifera of Different Evolutionary Branches
N. V. Ostroverkhova, Kucher An, N. Babushkina, O. L. Konusova, I. Sharakhov
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引用次数: 2
Abstract
An assessment of the genetic diversity of the microsatellite locus mrjp3 in honeybee of European Apis mellifera subspecies was conducted. Differences in the frequency of alleles for the mrjp3 locus were found in honeybees of different evolutionary branches. Allele "529" was found to be specific for the Apis mellifera mellifera, evolutionary branch M, while alleles "406", "518", and "485" were characteristic of the southern subspecies of bees (A. m. carnica, A. m. carpatica (some researchers consider this breed as a derivative of A. m. carnica), and A. m. caucasica; evolutionary branches C and O, respectively). A high correspondence of the studied nucleotide sequences (>=99% identity) with the reference sequences (Genbank) was established indicating a high conservation of the repetitive region of the mrjp3 gene in A. mellifera subspecies inhabiting different geographic regions of Europe and Siberia. Locus mrjp3 is of considerable interest for further study as a candidate marker for differentiation of bee subspecies.
对欧洲蜜蜂亚种微卫星位点mrjp3的遗传多样性进行了评价。在不同进化分支的蜜蜂中发现mrjp3位点的等位基因频率存在差异。等位基因“529”是蜜蜂进化分支“蜜蜂蜜蜂”所特有的,而等位基因“406”、“518”和“485”是南方亚种蜜蜂(a . M . carnica)、a . M . carpatica(一些研究者认为该品种是a . M . carnica的衍生物)和a . M . caucasica)所特有的;进化分支C和O分别)。所研究的核苷酸序列与参考序列(Genbank)高度对应(>=99%),表明在生活在欧洲和西伯利亚不同地理区域的A. mellifera亚种中mrjp3基因的重复区域具有高度保守性。mrjp3基因座作为蜜蜂亚种分化的候选标记,值得进一步研究。