13-cis-Retinoic Acid Affects Brain Perfusion and Function: In Vivo Study

IF 2.8 4区医学 Q2 Medicine

Molecular Imaging Pub Date : 2023-02-13 DOI:10.1155/2023/7855924

Fatma J Al-Saeedi, P. Rajendran

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Abstract

Purpose. Study the effects of 13-cis-retinoic acid (13-RA), a synthetic analogue of a vitamin A used for the treatment of severe acne, on the blood flow in the rat brain using technetium-99m hexamethyl propylene amine oxime (99mTc-HMPAO) imaging. Methods. A total of 30 adult male Wistar rats were divided into the control (C), low-dose (L), and high-dose (H) groups. The L and H rats were exposed subcutaneously to 0.3 and 0.5 mg, respectively, of 13-RA per kg of body weight for seven days. Brain blood flow imaging was performed using a gamma camera. Then, a region of interest (ROI) around the brain (target, T), a whole-body region (WB), and a background region (BG) was selected and delimited. The net 99mTc-HMPAO brain counts were calculated as the net target counts, NTC = T − BG / WB − BG in all groups. At the end of the 99mTc-HMPAO brain blood flow imaging, the brain, heart, kidney, lung, and liver were rapidly removed, and their uptake was determined. Brain histopathological analysis was performed using hematoxylin and eosin stains. In addition, the plasma fatty acids were studied using gas chromatography/mass spectrometry. Results. There were highly significant differences between L and H in comparison to C and across the groups. The 99mTc-HMPAO radioactivity in the brain showed increased uptake in a dose-dependent manner. There were also significant changes in the brain tissues and decreased free fatty acids among the groups compared to C. Conclusion. 13-RA increases 99mTcHMPAO brain perfusion, uptake, and function and reduces fatty acids.

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13-顺式维甲酸对脑灌注和功能的影响：体内研究

意图使用99mTc-HMPAO显像研究13-顺式视黄酸（13-RA）对大鼠脑血流的影响，13-RA是一种用于治疗严重痤疮的维生素a的合成类似物。方法。将30只成年雄性Wistar大鼠分为对照组（C）、低剂量组（L）和高剂量组（H）。L和H大鼠皮下暴露于0.3和0.5 毫克13-RA每公斤体重，持续7天。使用伽马照相机进行脑血流成像。然后，选择并界定大脑周围的感兴趣区域（ROI）（目标，T）、全身区域（WB）和背景区域（BG）。计算所有组的99mTc-HMPAO脑净计数作为净靶计数，NTC=T−BG/WB−BG。在99mTc-HMPAO脑血流成像结束时，迅速取出脑、心、肾、肺和肝，并测定其摄取量。使用苏木精和伊红染色进行脑组织病理学分析。此外，采用气相色谱/质谱法对血浆脂肪酸进行了研究。后果与C相比，L和H之间以及各组之间存在非常显著的差异。大脑中的99mTc-HMPAO放射性以剂量依赖的方式显示摄取增加。与C相比，各组脑组织也发生了显著变化，游离脂肪酸减少。结论。13-RA增加99mTcHMPAO脑灌注、摄取和功能，并减少脂肪酸。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Imaging 生物-核医学

CiteScore

4.50

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： Molecular Imaging is a peer-reviewed, open access journal highlighting the breadth of molecular imaging research from basic science to preclinical studies to human applications. This serves both the scientific and clinical communities by disseminating novel results and concepts relevant to the biological study of normal and disease processes in both basic and translational studies ranging from mice to humans.