Analysis of the Bacterial and Fungal Community Profiles in Bulk Soil and Rhizospheres of Three Mungbean [Vigna radiata (L.) R. Wilczek] Genotypes through PCR-DGGE

IF 0.3
Anna Reyes, E. Ocampo, M. C. Manuel, B. Mendoza
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引用次数: 3

Abstract

Each plant species is regarded to substantially influence and thus, select for specific rhizosphere microbial populations. This is considered in the exploitation of soil microbial diversity associated with important crops, which has been of interest in modern agricultural practices for sustainable productivity. This study used PCR-DGGE (polymerase chain reaction - denaturing gradient gel electrophoresis) in order to obtain an initial assessment of the bacterial and fungal communities associated in bulk soil and rhizospheres of different mungbean genotypes under natural field conditions. Integrated use of multivariate analysis and diversity index showed plant growth stage as the primary driver of community shifts in both microbial groups while rhizosphere effect was found to be less discrete in fungal communities. On the other hand, genotype effect was not discerned but not inferred to be absent due to possible lack of manifestations of differences among genotypes based on tolerance to drought under non-stressed environment, and due to detection limits of DGGE. Sequence analysis of prominent members further revealed that Bacillus and Arthrobacter species were dominant in bacterial communities whereas members of Ascomycota and Basidiomycota were common in fungal communities of mungbean. Overall, fungal communities had higher estimated diversity and composition heterogeneity, and were more dynamic under plant growth influence, rhizosphere effect and natural environmental conditions during mungbean growth in upland field. These primary evaluations are prerequisite to understanding the interactions between plant and rhizosphere microorganisms with the intention of employing their potential use for sustainable crop production.
3种绿豆块状土壤和根际细菌、真菌群落特征分析PCR-DGGE基因分型方法[j]
每一种植物都被认为对特定的根际微生物种群具有重大影响,因此可以选择特定的根际微生物种群。在开发与重要作物相关的土壤微生物多样性时考虑到了这一点,这在现代农业实践中对可持续生产力很感兴趣。本研究采用PCR-DGGE(聚合酶链反应-变性梯度凝胶电泳)技术,对自然大田条件下不同绿豆基因型土壤和根际细菌和真菌群落进行初步评估。多变量分析和多样性指数综合分析表明,植物生长阶段是两种微生物群落转移的主要驱动因素,而真菌群落的根际效应离散性较小。另一方面,由于在非胁迫环境下,基因型之间的耐旱性差异可能没有表现出来,而且DGGE的检测范围有限,因此没有发现基因型效应,但也不能推断基因型效应不存在。结果表明,绿豆真菌群落中以芽孢杆菌和节菌属为主,而子囊菌属和担子菌属较多。总体而言,绿豆旱田真菌群落在植物生长、根际效应和自然环境条件下具有较高的多样性和组成异质性,且更具活力。这些初步评价是了解植物和根际微生物之间相互作用的先决条件,目的是利用它们在可持续作物生产中的潜在用途。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International Letters of Natural Sciences
International Letters of Natural Sciences MULTIDISCIPLINARY SCIENCES-
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