Assessment of Genetic Variation of the Genus Gymnema in Vietnam, Using RAPD and ITS-rDNA Markers

IF 1.2 Q4 PHARMACOLOGY & PHARMACY

Journal of Herbs, Spices and Medicinal Plants Pub Date : 2022-03-30 DOI:10.1080/10496475.2022.2058672

Pham Ha Thanh Tung, Tran Van on, Phung Thanh Huong

引用次数: 3

Abstract

ABSTRACT Gymnema is a genus of the Apocynaceae with more than 50 species. Some of its species such as G. sylvestre and G. latifolium have been reported for their medicinal value. In this study, the genetic diversity of 20 samples of four species of Gymnema in Vietnam was evaluated using the Random Amplification of Polymorphic DNA (RAPD) and the internal transcribed spacer of nuclear ribosomal DNA (ITS-rDNA). Amplified bands obtained with primers UBC701, UBC709, and OPC4 were the highest polymorphic in RAPD analysis. The phylogenetic trees were built grouping the 20 samples into four clusters corresponding to the four Gymnema species according to morphological classification. A number of characteristic SNPs were identified for each species which can be used for authentication and differentiation. The results showed the consensus and usefulness of the two barcoding techniques in the molecular identification and characterization of the Gymnema genus.

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用RAPD和ITS rDNA标记评价越南裸泳属的遗传变异

Gymnema是夹竹桃科下的一个属，有50多种。它的一些物种，如G.sylvestre和G.latifolium已被报道具有药用价值。本研究采用多态DNA随机扩增技术（RAPD）和核核糖体DNA内部转录间隔区技术（ITS rDNA）对越南4种裸藻的20个样品的遗传多样性进行了评价。引物UBC701、UBC709和OPC4扩增出的扩增带多态性最高。建立了系统发育树，根据形态学分类将20个样本分为四个聚类，对应于四个Gymnema物种。为每个物种鉴定了许多可用于鉴定和分化的特征SNP。结果表明，这两种条形码技术在Gymnema属的分子鉴定和鉴定中具有一致性和实用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Herbs, Spices and Medicinal Plants Medicine-Complementary and Alternative Medicine

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.