Effectiveness of Combination of Chitosan Gel and Hydroxyapatite from Crabs Shells (Portunus pelagicus) Waste as Bonegraft on Periodontal Network Regeneration through IL-1 and BMP-2 Analysis

IF 3 Q3 MATERIALS SCIENCE, BIOMATERIALS
Asdar Gani, R. Yulianty, Supiaty Supiaty, Machirah Rusdy, Gustivanny Dwipa Asri, Dian Eka Satya, Ayu Rahayu Feblina, H. Achmad
{"title":"Effectiveness of Combination of Chitosan Gel and Hydroxyapatite from Crabs Shells (Portunus pelagicus) Waste as Bonegraft on Periodontal Network Regeneration through IL-1 and BMP-2 Analysis","authors":"Asdar Gani, R. Yulianty, Supiaty Supiaty, Machirah Rusdy, Gustivanny Dwipa Asri, Dian Eka Satya, Ayu Rahayu Feblina, H. Achmad","doi":"10.1155/2022/1817236","DOIUrl":null,"url":null,"abstract":"Background Periodontitis can be treated by regenerating periodontal tissue using a bone graft. Several natural materials such as chitosan and minerals such as hydroxyapatite can be developed to increase periodontal tissue regeneration. Chitosan has a high potential in healing wounds. Hydroxyapatite has excellent properties such as biocompatibility, osteoconductive, osteoinductive, and osteogenesis, making it an ideal material for soft and hard tissue regeneration. Chitosan and hydroxyapatite can be obtained from the shells of crustaceans, such as crabs shells (Portunus pelagicus). Objective To assess the effectiveness of the combination of chitosan gel and hydroxyapatite powder as a bone graft on periodontal tissue regeneration in experimental animals. Periodontal tissue regeneration was assessed by expressing inflammatory cytokine gene indicators IL-1 and BMP-2. Methods Experimental laboratory research and clinical trials with posttest only control group design. Twenty-seven Wistar rats were divided into three groups. Then the femoral bone defect was made, the positive control group was given placebo gel, the positive control group was given BATAN hydroxyapatite, and the test group was given a combination of chitosan gel and hydroxyapatite crab shells. Wistar rats were sacrificed on days 7, 14, and 21, and the femur bone was then taken for immunohistochemical analysis to determine the levels of IL-1 and BMP-2. The Kolmogorov–Smirnov test, Levene test, and one-way ANOVA analyzed the data. Results On days 7, 14, and 21, the expression levels of IL-1 and BMP2 were significantly different between the three groups. The group added with chitosan gel and crab shell HA showed a faster decrease in IL-1 expression than the control group. BMP-2 expression increased in the test group compared to the control group. Conclusion The combination of chitosan gel and hydroxyapatite inhibited the production of proinflammatory cytokines and increased the production of BMP-2.","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2022-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biomaterials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2022/1817236","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 4

Abstract

Background Periodontitis can be treated by regenerating periodontal tissue using a bone graft. Several natural materials such as chitosan and minerals such as hydroxyapatite can be developed to increase periodontal tissue regeneration. Chitosan has a high potential in healing wounds. Hydroxyapatite has excellent properties such as biocompatibility, osteoconductive, osteoinductive, and osteogenesis, making it an ideal material for soft and hard tissue regeneration. Chitosan and hydroxyapatite can be obtained from the shells of crustaceans, such as crabs shells (Portunus pelagicus). Objective To assess the effectiveness of the combination of chitosan gel and hydroxyapatite powder as a bone graft on periodontal tissue regeneration in experimental animals. Periodontal tissue regeneration was assessed by expressing inflammatory cytokine gene indicators IL-1 and BMP-2. Methods Experimental laboratory research and clinical trials with posttest only control group design. Twenty-seven Wistar rats were divided into three groups. Then the femoral bone defect was made, the positive control group was given placebo gel, the positive control group was given BATAN hydroxyapatite, and the test group was given a combination of chitosan gel and hydroxyapatite crab shells. Wistar rats were sacrificed on days 7, 14, and 21, and the femur bone was then taken for immunohistochemical analysis to determine the levels of IL-1 and BMP-2. The Kolmogorov–Smirnov test, Levene test, and one-way ANOVA analyzed the data. Results On days 7, 14, and 21, the expression levels of IL-1 and BMP2 were significantly different between the three groups. The group added with chitosan gel and crab shell HA showed a faster decrease in IL-1 expression than the control group. BMP-2 expression increased in the test group compared to the control group. Conclusion The combination of chitosan gel and hydroxyapatite inhibited the production of proinflammatory cytokines and increased the production of BMP-2.
白细胞介素1和骨形成蛋白-2分析壳聚糖凝胶和废弃蟹壳羟基磷灰石复合骨痂对牙周网络再生的影响
牙周炎可以通过骨移植再生牙周组织来治疗。几种天然材料,如壳聚糖和矿物质,如羟基磷灰石,可以开发促进牙周组织再生。壳聚糖在伤口愈合方面具有很高的潜力。羟基磷灰石具有生物相容性、骨导电性、骨诱导性和成骨性等优良性能,是软硬组织再生的理想材料。壳聚糖和羟基磷灰石可以从甲壳类动物的壳中获得,如蟹壳(Portunus pelagicus)。目的探讨壳聚糖凝胶与羟基磷灰石粉复合植骨对实验性动物牙周组织再生的影响。通过表达炎症细胞因子基因指标IL-1和BMP-2来评估牙周组织再生。方法采用单纯后测对照组设计,进行实验室实验研究和临床试验。将27只Wistar大鼠分为三组。然后制作股骨缺损,阳性对照组给予安慰剂凝胶,阳性对照组给予巴丹羟基磷灰石,试验组给予壳聚糖凝胶与羟基磷灰石蟹壳的联合治疗。Wistar大鼠于第7、14、21天处死,取股骨进行免疫组化分析,测定IL-1和BMP-2水平。采用Kolmogorov-Smirnov检验、Levene检验和单因素方差分析对数据进行分析。结果在第7、14、21天,三组间IL-1、BMP2的表达水平均有显著差异。壳聚糖凝胶组和蟹壳HA组IL-1表达下降速度明显快于对照组。与对照组相比,试验组BMP-2表达升高。结论壳聚糖凝胶与羟基磷灰石联合使用可抑制促炎细胞因子的产生,增加BMP-2的产生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
International Journal of Biomaterials
International Journal of Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
4.30
自引率
3.20%
发文量
50
审稿时长
21 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信