A chromatin accessibility landscape during early adipogenesis of human adipose-derived stem cells

IF 3.5 4区生物学 Q2 ENDOCRINOLOGY & METABOLISM

Adipocyte Pub Date : 2022-04-17 DOI:10.1080/21623945.2022.2063015

Sen Li, Xiaolin Zong, Liheng Zhang, Luya Li, Jianxin Wu

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引用次数: 3

Abstract

ABSTRACT Obesity has become a serious global public health problem; a deeper understanding of systemic change of chromatin accessibility during human adipogenesis contributes to conquering obesity and its related diseases. Here, we applied the ATAC-seq method to depict a high-quality genome‐wide time-resolved accessible chromatin atlas during adipogenesis of human adipose-derived stem cells (hASCs). Our data indicated that the chromatin accessibility drastic dynamically reformed during the adipogenesis of hASCs and 8 h may be the critical transition node of adipogenesis chromatin states from commitment phase to determination phase. Moreover, upon adipogenesis, we also found that the chromatin accessibility of regions related to anti-apoptotic, angiogenic and immunoregulatory gradually increased, which is beneficial to maintaining the health of adipose tissue (AT). Finally, the chromatin accessibility changed significantly in intronic regions of peroxisome proliferator‐activated receptor γ during adipogenesis, and these regions were rich in transcription factors binding motifs that were exposed for further regulation. Overall, we systematically analysed the complex change of chromatin accessibility occurring in the early stage of adipogenesis and deepened our understanding of human adipogenesis. Furthermore, we also provided a good reference data resource of genome‐wide chromatin accessibility for future studies on human adipogenesis.

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人类脂肪干细胞早期脂肪生成过程中的染色质可及性景观

肥胖已成为严重的全球性公共卫生问题;深入了解人类脂肪形成过程中染色质可及性的系统性变化有助于战胜肥胖及其相关疾病。在这里，我们应用ATAC-seq方法来描绘人类脂肪来源干细胞(hASCs)脂肪形成过程中高质量的全基因组时间分辨率可访问的染色质图谱。我们的数据表明，在hASCs的脂肪形成过程中，染色质可及性发生了剧烈的动态改变，8 h可能是脂肪形成染色质状态从承诺期到决定期的关键过渡节点。此外，在脂肪形成过程中，我们还发现抗凋亡、血管生成和免疫调节相关区域的染色质可及性逐渐增加，这有利于维持脂肪组织(AT)的健康。最后，在脂肪形成过程中，过氧化物酶体增殖物激活受体γ内含子区域的染色质可及性发生了显著变化，这些区域富含转录因子结合基序，可以进一步调控。总的来说，我们系统地分析了脂肪形成早期染色质可及性的复杂变化，加深了我们对人类脂肪形成的认识。此外，我们还为未来人类脂肪形成的全基因组染色质可及性研究提供了良好的参考数据资源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Adipocyte Medicine-Histology

CiteScore

6.50

自引率

3.00%

发文量

审稿时长

32 weeks

期刊介绍： Adipocyte recognizes that the adipose tissue is the largest endocrine organ in the body, and explores the link between dysfunctional adipose tissue and the growing number of chronic diseases including diabetes, hypertension, cardiovascular disease and cancer. Historically, the primary function of the adipose tissue was limited to energy storage and thermoregulation. However, a plethora of research over the past 3 decades has recognized the dynamic role of the adipose tissue and its contribution to a variety of physiological processes including reproduction, angiogenesis, apoptosis, inflammation, blood pressure, coagulation, fibrinolysis, immunity and general metabolic homeostasis. The field of Adipose Tissue research has grown tremendously, and Adipocyte is the first international peer-reviewed journal of its kind providing a multi-disciplinary forum for research focusing exclusively on all aspects of adipose tissue physiology and pathophysiology. Adipocyte accepts high-profile submissions in basic, translational and clinical research.