Effects of fostriecin on β2-adrenoceptor-driven responses in human mast cells

IF 2.4 4区医学 Q3 TOXICOLOGY

Journal of Immunotoxicology Pub Date : 2017-01-01 DOI:10.1080/1547691X.2016.1259277

R. Bastan, N. Eskandari, Hamidrez J Ardakani, Peter T Peachell

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引用次数: 2

Abstract

Abstract As part of the intracellular processes leading to mast cell and basophil activation, phosphorylation of key substrates is likely to be important. These processes, mediated by phosphatases, are responsible for regulating phosphorylation. The aim of the present study was to determine effects fostriecin – a selective inhibitor of PP2A (protein phosphatase-2) – on β2-adrenoceptor-driven responses in human mast cells. Here, the effects of fostriecin (PP inhibitors) on the inhibition of histamine release from HLMC, on β-adrenoceptor-driven responses in mast cells and on desensitization were investigated. Long-term incubation (24 h) of mast cells with fostriecin (10−6 M) resulted in a significant (p < 0.001) reduction in the maximal response (from 41.2 [± 3.0] to 29.9 [± 4.2] %) to salbutamol following fostriecin treatment. The results showed that fostriecin pretreatment significantly attenuated the inhibitory effects of salbutamol. Overall, the present study suggested that PP2A has an important role in regulating mast cell β2-adrenoceptors.

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磷三烯星对人肥大细胞β2-肾上腺素受体驱动反应的影响

作为导致肥大细胞和嗜碱性粒细胞活化的细胞内过程的一部分，关键底物的磷酸化可能很重要。这些过程由磷酸酶介导，负责调节磷酸化。本研究的目的是确定fostriecin -一种选择性PP2A(蛋白磷酸酶-2)抑制剂-对人肥大细胞β2-肾上腺素受体驱动反应的影响。本研究考察了fostriecin (PP抑制剂)对HLMC组胺释放抑制、肥大细胞β-肾上腺素受体驱动反应和脱敏的影响。肥大细胞与福氏菌素(10−6 M)长期孵卵(24 h)后，福氏菌素对沙丁胺醇的最大应答率(从41.2[±3.0]%降至29.9[±4.2]%)显著(p < 0.001)降低。结果表明，乳酸菌素预处理能显著减弱沙丁胺醇的抑制作用。综上所述，本研究提示PP2A在调节肥大细胞β2-肾上腺素受体中具有重要作用。

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来源期刊

Journal of Immunotoxicology 医学-毒理学

CiteScore

6.70

自引率

3.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Immunotoxicology is an open access, peer-reviewed journal that provides a needed singular forum for the international community of immunotoxicologists, immunologists, and toxicologists working in academia, government, consulting, and industry to both publish their original research and be made aware of the research findings of their colleagues in a timely manner. Research from many subdisciplines are presented in the journal, including the areas of molecular, developmental, pulmonary, regulatory, nutritional, mechanistic, wildlife, and environmental immunotoxicology, immunology, and toxicology. Original research articles as well as timely comprehensive reviews are published.