Antidepressant, neuropharmacological activity and mode of action of theaflavin-3-gallate in in vitro and in vivo models of depression

IF 0.9 4区医学 Q4 PHARMACOLOGY & PHARMACY

Bangladesh Journal of Pharmacology Pub Date : 2018-11-21 DOI:10.3329/BJP.V13I4.37773

Ying Wang, J. Chen, Jing Wang, Xin Guo, Ning Wang, Jie Yuan

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引用次数: 2

Abstract

Herein the antidepressant-like effect of theaflavin-3-gallate, a plant polyphenolic compound in 1-methyl-4-phenyl pyridinium-induced neuronal injury in N2a cells used as an in vitro model of depression as well as in vivo. The results from MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) and lactic dehydrogenase assay revealed that theaflavin-3-gallate exerted a neuroprotective effect in N2a cells. Treatments with mixtures of 1-methyl-4-phenyl pyridinium (20 µM) and four concentration levels of theaflavin-3-gallate increased cell viability from 23.3 to 79.6% while lactic dehydrogenase leakage value decreased from 63.2 to 23.4 U/L. Fura-2-acetoxymethyl ester assay for Ca2+ concentration measurement revealed that different doses of theaflavin-3-gallate or fluoxetine (20 µM) reduced the intracellular Ca2+ overloading in N2a cells induced by 1-methyl-4-phenyl pyridinium. Inverted phase microscopy showed that theaflavin-3-gallate induced neurite outgrowth in 1-methyl-4-phenyl pyridinium-induced neurotoxicity in N2a cells. Theaflavin-3-gallate could significantly reduce the immobility time of mice in both forced swimming test and tail suspension test.Video Clip of Methodology:MTT Assay: 3 min 38 sec Full Screen Alternate

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茶黄素-3-没食子酸酯在体外和体内抑郁症模型中的抗抑郁、神经药理活性和作用方式

本文研究了茶黄素-3-没食子酸酯(一种植物多酚化合物)在1-甲基-4-苯基吡啶诱导的N2a细胞神经损伤中的抗抑郁样作用，并将其作为体外和体内抑郁模型。MTT(3-(4,5 -二甲基噻唑-2-基)- 2,5 -二苯基溴化四唑)和乳酸脱氢酶测定结果显示茶黄素-3-没食子酸酯对N2a细胞具有神经保护作用。1-甲基-4-苯基吡啶(20µM)与4种浓度的茶黄素-3-没食子酸酯混合处理使细胞活力从23.3提高到79.6%，乳酸脱氢酶渗漏值从63.2降低到23.4 U/L。fura -2-乙酰氧基甲酯法测定Ca2+浓度发现，不同剂量的茶黄素-3-没食子酸酯或氟西汀(20µM)可降低1-甲基-4-苯基吡啶诱导的N2a细胞内Ca2+超载。倒置相显微镜显示，茶黄素-3-没食子酸酯诱导1-甲基-4-苯基吡啶诱导的N2a细胞神经毒性神经突生长。茶黄素-没食子酸酯在强迫游泳和悬尾实验中均能显著减少小鼠的静止时间。方法视频剪辑:MTT测定:3分38秒全屏交替

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来源期刊

Bangladesh Journal of Pharmacology PHARMACOLOGY & PHARMACY-

CiteScore

1.50

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is an open access, video component, peer-reviewed journal of the Bangladesh Pharmacological Society (BDPS). A scholarly publication is defined ''open access'' when there are no financial, legal or technical barriers to accessing it. Anyone can read, download, copy, distribute, print or use it in educational purpose within the legal agreements. Peer review of a manuscript means the evaluation of research work by one or more people with similar competences. It can be open or blind. We do the single-blind peer review. Our readers want to know the methodology used by the researchers which is very important to get the results. The video clip of one of the methodologies is prepared by the authors and submit it to publish.