Solid State Fermentation of Groundnut Shell by Schizophyllum commune BCC26414 for Production of Cellobiose Dehydrogenase

Abstract

Cellobiose dehydrogenase (CDH) enzyme is secreted extracellularly by wood-rotting fungi of the phyla Basidiomycetes and Ascomycetes. The reducing ends of cellobiose, lactose, and maltose are oxidized by CDH to produce their respective lactones. These lactones are consequently converted into their carboxylic acids such as cellobionic acid, lactobionic acid, and maltobionic acid. Due to its commercial unavailability and its applications in various fields, there is a need for cost-effective CDH production. In the present work, Schizophyllum commune BCC26414 has been used for CDH production by solid-state fermentation (SSF). CDH production was optimized by one factor at a time (OFAT) approach in terms of initial moisture content, inoculum size, incubation temperature, particle size, and fermentation time. BBD (Box-Behnken Design) was used to perform statistical optimization of CDH production using statistical software, Response Surface Methodology (RSM) Maximum CDH production was obtained when groundnut shell was used as a substrate at 30°C on 9th day of incubation, with 0.5mm to 1mm particle size, 2 ml inoculum size, and the initial moisture content 50% using Schizophyllum commune BCC26414. RSM enhances enzyme production to 1.6-fold as compared to unoptimized conditions. This is the first report on solid-state CDH production using groundnut shells as solid substrate. A variety of CDH applications have been reported in the fields of biomedical, biocatalysts, bioremediation, and biosensors. This study will be helpful in the cost-effective production of CDH for various applications.