METHODS AND RESULTS OF STUDYING THE STRUCTURE AND TINCTORIAL PROPERTIES OF CATGUT THREAD

O. Maksymenko, V. Hryn
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Abstract

Suture material used in abdominal surgery must have more or less antigenic properties so that during wound healing it could be destroyed by an immune response to rejection, which is known to involve cytotoxic T-lymphocytes and macrophages. This, in turn, can lead to the development of aseptic peritonitis. Therefore, while planning an experimental simulation of aseptic peritonitis, we decided to use catgut thread as an antigenic stimulation of the local immune system. The aim of our research was to study the morphological criteria of the microscopic structure and tinctorial properties of catgut thread. The material of the research was the sterile catgut thread sized 2/0, 0.3 mm thick, of equal lengths, which we twisted arbitrarily into flat compact globules occupying an area of approximately 1 cm2. In order to carry out a histological examination, some catgut globules were subjected to total staining with a hematoxylineosin solution and subsequent embedding of these globules in a paraffin block and making appropriate sections from it. The other half of the catgut globules were studied using the method of epoxy plastination with the production of grinds with staining by a 1% solution of methylene blue on a 1% borax and without any staining. After the total staining of the catgut globules with a hematoxylin-eosin solution, a clear acidophilic reaction was revealed, which was manifested in the intense pink color. Catgut thread has a non-uniform fibrous structure, in which dark streaks stand out, dividing it into a series of longitudinal, lighter, layered fascicles, which consist of a dense collection of thin fibrillar elements. These fibrillar elements are orderly fascicles of collagen fibers, while the dark streaks are layers of loose fibrous connective tissue. These structures can objectively be indicative of morphological criteria in the microscopic analysis of destructive changes of a catgut implant in the peritoneal cavity in an experimental animal.
研究羊线的结构和着色性能的方法与结果
用于腹部手术的缝合材料必须具有或多或少的抗原性,以便在伤口愈合过程中,它可以被免疫反应破坏,这是已知的涉及细胞毒性t淋巴细胞和巨噬细胞。这反过来又可导致无菌性腹膜炎的发展。因此,在计划无菌性腹膜炎的实验模拟时,我们决定使用肠线作为局部免疫系统的抗原刺激。本研究的目的是研究羊线的显微结构和染色性能的形态学标准。研究的材料是无菌羊线,直径为0.2毫米,长度相等,我们将其任意扭曲成扁平致密的球体,占地面积约为1平方厘米。为了进行组织学检查,将一些肠球用苏木精溶液进行全染色,然后将这些球包埋在石蜡块中并从中制作适当的切片。另一半的线球采用环氧塑化的方法进行研究,用1%的亚甲基蓝溶液在1%硼砂上染色,不染色。苏木精-伊红溶液全染色后,可见明显的亲酸反应,呈深粉红色。羊线具有不均匀的纤维结构,其中深色条纹突出,将其分成一系列纵向的、较轻的、分层的束,这些束由密集的细纤维元素组成。这些纤维成分是有序的胶原纤维束,而深色条纹是疏松的纤维结缔组织层。这些结构可以客观地指示在实验动物腹膜腔中肠线植入物的破坏性变化的显微分析中的形态学标准。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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