Role of the alkylglycerone phosphate synthase in isoproterenol-induced cardiac hypertrophy

国际生物医学工程杂志 Pub Date : 2019-08-28 DOI:10.3760/CMA.J.ISSN.1673-4181.2019.04.004

Yijie Liu, Qiaoman Fei, Bingyan Cao, Manman Qiu, Huan Huang, Jiaxin Song, Bing Yang, Ling Zhang

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Abstract

Objective To research the effect of alkylation of glycerol phosphate synthase(AGPS) in isoproterenol (ISO) induced rat cardiac hypertrophy. Methods The pathological cardiac hypertrophy rat model was constructed by ISO intraperitoneal injection. Twelve healthy Sprague-Dawley rats (120~150 g) were divided into ISO group and control group randomly. In the ISO group, rats were injected with ISO (3 mg/kg) per day for two consecutive weeks. In the control group, rats were injected with normal saline (3 mg/kg) per day for two consecutive weeks. Changes of left ventricular diastolic diameter, left ventricular posterior wall thickness, left ventricular ejection fraction, left ventricular short-axis shortening rate and left ventricular mass were detected by echocardiography. The cross-sectional area of myocardial cells in rats was measured by hematoxylin-eosin staining. The expression of hypertrophic factors [atrial natriuretic peptide (ANP), myosin light chain-2V (MLC-2V), α-myosin heavy chain (α-MHC)] and AGPS were detected by Western Blot and real-time quantitative PCR (qPCR). Results The results of echocardiography showed that the cardiac hypertrophy rat model was successfully constructed. The results of hematoxylin-eosin staining showed that the myocardial cross-sectional area in the ISO group was significantly larger than that of the control group. The Western Blot and qPCR results indicated that the relative expression of protein and mRNA of hypertrophic factor and AGPS in the ISO group were both up-regulated comparing with that of the control group, and the differences were statistical significance (all P<0.05). Conclusions The rat model of pathological cardiac hypertrophy with up-regulated AGPS expression was successfully constructed providing a theoretical basis for further study on the role of AGPS in pathogenesis of pathological cardiac hypertrophy. Key words: Cardiomyopathy, hypertrophic; Isoproterenol; Alkylglycerone phosphate synthase

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烷基甘油磷酸合成酶在异丙肾上腺素诱导的心肌肥大中的作用

目的研究甘油磷酸合成酶（AGPS）烷基化在异丙肾上腺素（ISO）诱导的大鼠心肌肥大中的作用。方法采用ISO腹腔注射法建立病理性心肌肥大大鼠模型。12只健康Sprague-Dawley大鼠（120～150g）随机分为ISO组和对照组。在ISO组中，大鼠每天注射ISO（3 mg/kg），连续两周。在对照组中，大鼠每天注射生理盐水（3mg/kg），连续两周。超声心动图检测左心室舒张直径、左心室后壁厚度、左心室射血分数、左心室短轴缩短率和左心室质量的变化。苏木精-伊红染色法测定大鼠心肌细胞的横截面积。用Western Blot和实时定量PCR（qPCR）检测肥大因子[心钠素（ANP）、肌球蛋白轻链-2V（MLC-2V）、α-肌球蛋白重链（α-MHC）]和AGPS的表达。结果超声心动图结果表明，成功构建了大鼠心肌肥厚模型。苏木精-伊红染色结果显示，ISO组的心肌截面积明显大于对照组。Western Blot和qPCR结果表明，ISO组肥大因子和AGPS蛋白和mRNA的相对表达均较对照组上调，结论成功构建了AGPS表达上调的病理性心肌肥厚大鼠模型，为进一步研究AGPS在病理性心肌肥大发病机制中的作用提供了理论依据。关键词：心肌病、肥厚型；异丙烯醇；烷基甘油酮磷酸合成酶

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国际生物医学工程杂志

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