Cassava torrado-like virus encodes a gene that facilitates the mechanical transmission to Nicotiana benthamiana of Cassava virus X

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
J. Jimenez, A. Leiva, W. Cuellar
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引用次数: 0

Abstract

Cassava-torrado-like virus (CsTLV) is a bipartite single-stranded RNA virus belonging to the family Secoviridae. The virus has been reported in Brazil and Colombia, and is usually found in mixed infections, and in plants affected by Cassava Frogskin Disease (CFSD), an endemic cassava disease in the Americas. Genome analysis of CsTLV identifies a gene in RNA2 implicated in pathogen movement in other torradoviruses. This gene (RNA2-ORF1) and another one no related to virus movement (Maf/Ham1) were amplified by PCR and cloned into constructs under the 35S promoter of cauliflower mosaic virus (CaMV), which then were transfered to agrobacterium binary vectors. When agro-infiltrated in Nicotiana benthamiana plants, only RNA2-ORF1 had a positive effect on the mechanical inoculation of cassava virus X (CsVX), a potexvirus that has a low rate of mechanical infection in N. benthamiana. Efficiency of CsVX mechanical transmission was measured by the number of infected plants, presence of symptoms, and titers of CsVX as measured by ELISA, two weeks after infection. On average, CsVX could infect 2.3 times more plants when these were previously agro-infiltrated with A. tumefaciens binary vector encoding CsTLV RNA2-ORF1. We conclude that the novel secovirid CsTLV associated with leaf spot symptoms in cassava, encodes a gene that could enhance other viral infections in N. benthamiana. Further studies are required to elucidate this effect and its role in mixed infections, often observed in cassava plants affected by CFSD.
木薯torrado样病毒编码一个基因,该基因促进木薯病毒X向本氏烟草的机械传播
类木薯病毒(Cassava torrado like virus,CsTLV)是一种二分单链RNA病毒,属于次级病毒科。该病毒已在巴西和哥伦比亚报告,通常在混合感染中发现,并在受美洲流行木薯病木薯蛙皮病(CFSD)影响的植物中发现。CsTLV的基因组分析确定了RNA2中的一个基因,该基因与其他托拉病毒的病原体运动有关。用聚合酶链式反应扩增该基因(RNA2-ORF1)和另一个与病毒运动无关的基因(Maf/Ham1),并将其克隆到花椰菜花叶病毒(CaMV)35S启动子下的构建体中,然后将其转移到农杆菌二元载体上。当农业渗透到本氏烟草植物中时,只有RNA2-ORF1对机械接种木薯病毒X(CsVX)有积极作用,该病毒是一种在本氏烟草中机械感染率较低的潜在病毒。CsVX机械传播的效率通过感染植物的数量、症状的存在以及感染后两周通过ELISA测量的CsVX滴度来测量。平均而言,当以前用编码CsTLV RNA2-ORF1的根癌分枝杆菌二元载体对这些植物进行农业渗透时,CsVX可以感染2.3倍以上的植物。我们的结论是,与木薯叶斑症状相关的新型secovirid CsTLV编码一个基因,该基因可以增强本氏猪笼草的其他病毒感染。需要进一步的研究来阐明这种影响及其在混合感染中的作用,这种感染通常在受CFSD影响的木薯植物中观察到。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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