The Influence of Giving Tempe Flour Toward the Amount and Morphology of White Male Rat (Rattus norvegicus) Erythrocytes Wistar Strain was Affected by Cigarette Smoke

Berkala Kedokteran Pub Date : 2019-09-28 DOI:10.20527/JBK.V15I2.7128

I. M. S. Harsa

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Abstract

Abstract: Cigarette smoke was one of free radicals source for the body. Free radical contain in cigarette smoke were hydroxide radicals (•OH). Hydroxide radicals would cause erythrocytes damage. Antioxidants were substances that can prevent free radicals. Now, natural antioxidants source has developed, one of that is tempe, because of it contained antioxidants substances such as carotene, Vitamin E, isoflavon, and factor II antioxidants. This resource was for knowing the influence of giving tempe flour toward the amount and morphology of white male rat (Rattus norvegicus) erythrocytes wistar strain was affected by cigarette smoke. A pure experiment which was used the post test only-control group design method. Sample of the resource was white male rat wistar strain aged of 3-4 month as much 30 rats by the weight around 180-220 gram. The samples divided into 3 groups that were control group (K1), treatment group 1 (K2), and treatment group 2 (K3), each group consists of 10 rats. Control group was not given a special treatment, it only got a standard feed, treatment group 1 got special treatment and it was affected by cigarette smoke, furthermore, treatment group 2 got standard feed, it was affected by cigarette smoke and tempe. Analysis of data used one way anova experiment. The test showed the significance P-value = 0,036 that was <α (0,05). The result showed that there was the influence of giving tempe flour toward the amount and morphology of white male rat (Rattus norvegicus) erythrocytes wistar strain was affected by cigarette smoke. Keywords: cigarette smoke, free radicals, erythrocytes, antioxidants, tempe

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烟熏对褐家鼠(Rattus norvegicus)红血球Wistar株数量和形态的影响

摘要：吸烟是人体自由基的来源之一。香烟烟雾中含有的自由基是氢氧根（•OH）。氢氧根会引起红细胞损伤。抗氧化剂是可以防止自由基的物质。现在，天然抗氧化剂的来源已经发展起来，其中之一是tempe，因为它含有抗氧化剂，如胡萝卜素、维生素E、异黄酮和因子II抗氧化剂。该资料是为了了解添加天贝粉对香烟烟雾影响的白色雄性大鼠（褐家鼠）红细胞数量和形态的影响。纯实验采用纯试验后对照组设计方法。资源的样品是3-4月龄的白色雄性大鼠wistar品系，多达30只大鼠，重量约为180-220克。样品分为3组，即对照组（K1）、治疗组1（K2）和治疗组2（K3），每组10只大鼠。对照组不给予特殊处理，只给予标准饲料，处理组1给予特殊处理并受到香烟烟雾的影响，此外，处理组2给予标准饲料并受到香烟烟和温度的影响。数据分析采用单向方差分析实验。试验显示显著性P值=0036，即<α（0,05）。结果表明，添加天贝粉对白雄鼠（褐家鼠）红细胞的数量和形态有影响。香烟烟雾对wistar品系红细胞数量和形态均有影响。关键词：香烟烟雾，自由基，红细胞，抗氧化剂，tempe

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Berkala Kedokteran

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24 weeks