Optimized xylose isomerase uptake and expression level in Saccharomyces cerevisiae for improving ethanol production

Q2 Agricultural and Biological Sciences
Mei Zhang, Wenxiu Fan, Jingyue Wang, Limin Cao
{"title":"Optimized xylose isomerase uptake and expression level in Saccharomyces cerevisiae for improving ethanol production","authors":"Mei Zhang, Wenxiu Fan, Jingyue Wang, Limin Cao","doi":"10.26789/AEB.2018.01.007","DOIUrl":null,"url":null,"abstract":"The ability to engineer the yeast Saccharomyces cerevisiae to efficiently convert lignocellulosic biomass to ethanol remains a considerable challenge. Here, we propose a new reprogrammable strategy to optimize the expression level of the xylose isomerase (XI) gene with the induction of mutations in S . cerevisiae  to improve efficient ethanol production and productivity. We sought to fine-tune the xylose uptake and catabolism abilities of S. cerevisiae  during fermentation by improving efficiency of the xylose transporter, which was fused with four copies of the XI gene under the control of different promoters to obtain recombinant yeast strains. In fermentation experiments, the optimized strain CW9 cultured in yeast extract-peptone (YP) medium containing approximately 65 g/L glucose and 55 g/L xylose produced consistent ethanol yields of 0.45 g/g total sugar in about 72 h, which was close to 90% of the theoretical yield. These promising results indicate that strain CW9 is the best producer of ethanol from mixed sugar when synthetically regulating the xylose assimilation pathway. Overall, this study provides an optimal method to control XI expression levels to find better conditions for enhancing biofuel production.","PeriodicalId":36987,"journal":{"name":"Applied Environmental Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2018-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Environmental Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26789/AEB.2018.01.007","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 1

Abstract

The ability to engineer the yeast Saccharomyces cerevisiae to efficiently convert lignocellulosic biomass to ethanol remains a considerable challenge. Here, we propose a new reprogrammable strategy to optimize the expression level of the xylose isomerase (XI) gene with the induction of mutations in S . cerevisiae  to improve efficient ethanol production and productivity. We sought to fine-tune the xylose uptake and catabolism abilities of S. cerevisiae  during fermentation by improving efficiency of the xylose transporter, which was fused with four copies of the XI gene under the control of different promoters to obtain recombinant yeast strains. In fermentation experiments, the optimized strain CW9 cultured in yeast extract-peptone (YP) medium containing approximately 65 g/L glucose and 55 g/L xylose produced consistent ethanol yields of 0.45 g/g total sugar in about 72 h, which was close to 90% of the theoretical yield. These promising results indicate that strain CW9 is the best producer of ethanol from mixed sugar when synthetically regulating the xylose assimilation pathway. Overall, this study provides an optimal method to control XI expression levels to find better conditions for enhancing biofuel production.
优化酿酒酵母木糖异构酶吸收和表达水平以提高乙醇产量
改造酿酒酵母有效地将木质纤维素生物质转化为乙醇的能力仍然是一个相当大的挑战。提高乙醇生产效率和生产效率。在发酵实验中,优化后的菌株CW9在含有约65 g/L葡萄糖和55 g/L木糖的酵母提取物-蛋白胨(YP)培养基中培养,在约72 h内产生一致的乙醇产量为0.45 g/g总糖,接近理论产量的90%。这些结果表明,菌株CW9在合成调节木糖同化途径时是混合糖中乙醇的最佳生产者。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Applied Environmental Biotechnology
Applied Environmental Biotechnology Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
3.70
自引率
0.00%
发文量
2
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信