A citrus essential oil causes higher disturbance on the growth kinetics of Enterococcus faecalis than Lactobacillus rhamnosus

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-12-15 DOI:10.17268/sci.agropecu.2022.034

C. Ambrosio, A. C. Miano, E. Saldaña, E. M. Da Gloria

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引用次数: 0

Abstract

Essential oils (EOs) have turned a promising alternative to using antibiotics in poultry production due to their antimicrobial properties. EOs could effectively combat pathogenic bacteria affecting poultry. Particularly, Citrus EOs, a by-product of citrus processing industries, could be a feasible alternative to this end due to their vast availability in the global market. Enterococci are associated with intestinal and extra-intestinal infections in poultry, which can increase poultry mortality. On the other hand, Lactobacilli are beneficial bacteria inhabiting the poultry gut and have health-promoting effects. The aim of this study was to evaluate the antibacterial activity of a commercial citrus EO, Orange oil phase essence (OOPE), on Enterococcus faecalis and Lactobacillus rhamnosus as well as to determine OOPE chemical composition. Results showed that OOPE inhibited E. faecalis and L. rhamnosus at 14.8 mg/mL. However, the evaluation of OOPE effects on the growth kinetics parameters of both bacteria reveled that OOPE caused higher disturbances on the growth kinetics of E. faecalis than L. rhamnosus. OOPE significantly reduced the maximal culture density (A) and growth rate (µmax) and extended the lag phase duration (λ) of E. faecalis in a dose-dependent manner, while OOPE slightly extended λ and affected µmax of L. rhamnosus. OOPE at 3.70 mg/mL reduced A and µmax in ~87.34 and 90.2%, respectively, while increased λ 3.8 times of E. faecalis. OOPE at this concentration reduced µmax in 11.8% and extended λ 1.38 times of L. rhamnosus. Therefore, OOPE had a selective antibacterial activity, presenting higher activity on E. faecalis. Despite, limonene was identified as the major compound (87.22%) of OOPE, minor compounds such as trans-carveol could be involved in conferring the selective antibacterial activity of OOPE.

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与鼠李糖乳杆菌相比，柑橘精油对粪肠球菌生长动力学的干扰更大

由于其抗菌特性，精油(EOs)已成为家禽生产中使用抗生素的有希望的替代品。EOs能有效地对抗家禽致病菌。特别是柑橘加工工业的副产品柑橘EOs，由于其在全球市场上的广泛可用性，可能是一种可行的替代方案。肠球菌与家禽肠道和肠外感染有关，可增加家禽死亡率。另一方面，乳酸菌是栖息在家禽肠道中的有益菌，具有促进健康的作用。本研究旨在评价商品柑橘精油精油(橙油相香精)对粪肠球菌和鼠李糖乳杆菌的抑菌活性，并测定其化学成分。结果表明，在14.8 mg/mL浓度下，OOPE对粪肠杆菌和鼠李糖乳杆菌均有抑制作用。然而，对两种细菌生长动力学参数的影响评价显示，OOPE对粪肠杆菌生长动力学的干扰高于鼠李糖乳杆菌。OOPE显著降低粪肠杆菌的最大培养密度(A)和生长速率(µmax)，并呈剂量依赖性地延长滞后期持续时间(λ)，而OOPE略微延长鼠李糖的λ并影响µmax。3.70 mg/mL OOPE可使粪肠杆菌的A和µmax分别降低~ 87.34%和90.2%，而使粪肠杆菌的λ增加3.8倍。该浓度的OOPE使鼠李糖的µmax降低了11.8%，λ延长了1.38倍。因此，OOPE具有选择性抗菌活性，对粪肠杆菌具有较高的抑菌活性。尽管柠檬烯被鉴定为OOPE的主要化合物(87.22%)，但反式卡维罗等次要化合物可能参与赋予OOPE选择性抗菌活性。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.