Relationship between joint function and circadian rhythm in a rat model of knee osteoarthritis undergoing acupuncture and moxibustion at acupoints selected based on liver theory

Q4 Medicine

中国组织工程研究 Pub Date : 2021-05-18 DOI:10.3969/J.ISSN.2095-4344.3121

Z. Xiaohong, Li Bocun, Lin Jia, Peng Rui

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引用次数: 0

Abstract

摘要背景:膝骨关节炎发病机制复杂,近年来有研究发现昼夜节律与膝骨关节炎关系密切,而肝与昼夜节律有一定联系,从肝论治取穴针灸影响昼夜节律的研究鲜见。目的:探讨从肝论治针灸治疗膝骨关节炎对关节功能的影响及其与昼夜节律的关系。方法:随机将40只3月龄SPF级雄性SD大鼠分为正常组、模型组、常规取穴组和从肝论治取穴组,每组10只。除正常组外,其余3组均制备右膝关节炎模型造模;常规取穴组取足三里+内、外膝眼;从肝论治取穴组取阳陵泉+肝俞+太冲。从造模术后第1周开始,每周进行旷场实验、足底痛阈测试实验和右膝关节活动度测量;治疗结束后,苏木精-伊红染色观察软骨组织病理结构,RT-PCR与免疫荧光法检测软骨组织中Bmal1、Clock基因与蛋白的表达。结果与结论:1与正常组相比,模型组大鼠平均速度、总运动距离明显降低,休息时间明显延长(P < 0.05),足底痛阈明显降低(P < 0.05), 关节活动度下降(P < 0.05),组织学评分明显增高(P < 0.05),且软骨组织中Bmal1、Clock基因与蛋白的表达均明显下降(P < 0.01);2与模型组相比,常规取穴组和从肝论治取穴组各项指标均有改善(P < 0.05),且从肝论治取穴组各项指标改善更明显(P < 0.05);3提示从肝论治取穴可能通过影响软骨细胞昼夜节律,修复软骨组织,减轻膝骨关节炎疼痛,恢复关节功能。关键词:骨;膝关节;骨关节炎;针灸;取穴;从肝论治;昼夜节律;足底痛阈

查看原文本刊更多论文

基于肝脏理论选择穴位针灸治疗大鼠膝关节骨性关节炎模型关节功能与昼夜节律的关系

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来源期刊

中国组织工程研究 Medicine-Orthopedics and Sports Medicine

CiteScore

0.30

自引率

0.00%

发文量

61083

期刊介绍： Chinese Journal of Tissue Engineering Research (CJTER) is supervised by the Ministry of Health, and sponsored by the Chinese Association of Rehabilitation Medicine and the Editorial Board of CJTER. CJTER is publishing the latest progress in tissue engineering research.Our main sections include stem cells, tissue constructions, biomaterials, orthopedic implants,digital orthopedics,organ tissue and cell transplantation. Efficiency of publication: All manuscripts accepted will be reviewed within 1 month.Time from acceptance to publishing is 3 months for excellent manuscripts, and 6 months for normal manuscripts.