Ultrasound-assisted dispersive liquid-liquid microextraction approach for preconcentration of acyclovir as antiviral drug in dosage forms prior to spectrophotometric determination

IF 1.3 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Bulletin of the Chemical Society of Ethiopia Pub Date : 2023-06-30 DOI:10.4314/bcse.v37i5.2

Amal H. Al-Bagawi, Ragaa El Sheikh, Osama M.A. Salem, Ghada M. Abdel Fattah, Mohannad M. Garoub, Ayman A. Gouda

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Abstract

ABSTRACT. For acyclovir (ACV) determination in bulk and dosage forms, quick, sensitive, straightforward, and eco-friendly ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME)-based spectrophotometric method has been created and validated. ACV with 1,2-naphthoquine-4-sulfonate (NQS) react in alkaline medium to produce a yellow-colored product, which is the basis of the newly created method. Investigation and optimization were done on the crucial experimental variables influencing ACV's extraction effectiveness. At λmax = 495 nm, the minuscule organic droplets were detected. The linearity was present from 0.1 to 3.0 μg/mL under ideal circumstances, with a linear correlation coefficient of 0.9995. The detection limit was 0.03 μg/mL and quantification limit was 0.1 μg/mL. 22.50 was the enrichment factor. Relative standard deviation (RSD%) as accuracy at 2.0 μg/mL of ACV was 1.0%, with good recovery (99.30%). The developed UA-DLLME method was effectively used to determine the ACV in dosage forms, and the validation was evaluated. Results from the suggested technique for dosage forms and pure ACV were in excellent agreement with the results from the official method. KEY WORDS: Ultrasound-assissted, Dispersive liquid–liquid microextraction, Acyclovir, Spectrophotometry, Dosage forms Bull. Chem. Soc. Ethiop. 2023, 37(5), 1081-1092. DOI: https://dx.doi.org/10.4314/bcse.v37i5.2

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超声辅助分散液-液微萃取法在分光光度测定前预浓缩阿昔洛韦作为抗病毒药物的剂型

摘要建立了一种快速、灵敏、简便、环保的超声辅助分散液-液微萃取(UA-DLLME)分光光度法测定原药和剂型阿昔洛韦(ACV)的方法。ACV与1,2-萘醌-4-磺酸盐(NQS)在碱性介质中反应生成黄色产物，这是新方法的基础。对影响ACV提取效果的关键实验变量进行了研究和优化。在λmax = 495 nm处，检测到微小的有机液滴。在0.1 ~ 3.0 μg/mL范围内呈良好的线性关系，线性相关系数为0.9995。检测限为0.03 μg/mL，定量限为0.1 μg/mL。22.50为富集因子。在ACV浓度为2.0 μg/mL时，相对标准偏差(RSD%)为1.0%，回收率为99.30%。建立的UA-DLLME方法可有效测定制剂中的ACV，并进行了验证。所建议的剂型和纯ACV测定方法的结果与官方方法的结果完全一致。关键词:超声辅助，分散液液微萃取，阿昔洛韦，分光光度法，剂型化学。Soc。阿比西尼亚人。2023年,37 (5),1081 - 1092 . DOI: https://dx.doi.org/10.4314/bcse.v37i5.2

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来源期刊

Bulletin of the Chemical Society of Ethiopia 化学-化学综合

CiteScore

2.20

自引率

8.30%

发文量

113

审稿时长

6-12 weeks

期刊介绍： The Bulletin of the Chemical Society of Ethiopia (BCSE) is a triannual publication of the Chemical Society of Ethiopia. The BCSE is an open access and peer reviewed journal. The BCSE invites contributions in any field of basic and applied chemistry.