Green coffee extract (Coffea canephora) improved the intestinal barrier and slowed colorectal cancer progression and its associated inflammation in rats

IF 2.4 Q3 NUTRITION & DIETETICS

PharmaNutrition Pub Date : 2022-12-01 DOI:10.1016/j.phanu.2022.100314

Caroline Woelffel Silva , Keila Rodrigues Zanardi , Mariana Grancieri , Neuza Maria Brunoro Costa , Leonardo Oliveira Trivillin , Mirelle Lomar Viana , Pollyanna Ibrahim Silva , André Gustavo Vasconcelos Costa

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引用次数: 0

Abstract

Background and aim

Cancer is directly associated with inflammation and oxidative stress. Thanks to its antioxidant activity, green coffee may have anticarcinogenic effects. The objective of this study was to assess the effect of an aqueous green coffee extract on local colorectal morphophysiological and systemic inflammatory and oxidative changes in an animal model of colorectal cancer (CRC).

Methods

CRC was induced in male Wistar rats for 5 weeks by dimethylhydrazine. After 10 weeks of carcinogenesis, the rats were divided: Healthy Control (HC, without cancer induction and without extract), Colorectal Cancer (CRC, with cancer induction and without extract), Health Green Coffee (HGC, without cancer induction and with extract) and colorectal cancer treatment with Green Coffee (CGC, with cancer induction and with extract) groups. The data were analyzed using the Student’s t-test or ANOVA and the Newman-Keuls post-hoc test (p < 0.05).

Results

Green coffee extract was source of caffeine, chlorogenic acid, and trigonelline that had in silico interaction with NF-κB. This extract contributed to the intestinal barrier integrity by decreasing lactulose and mannitol urinary excretion, increasing fecal IgA levels and reducing malignant tumors in the colon and rectum. In addition, it reduced the systemic production of the inflammatory cytokines TNFα and IL-6. However, green coffee extract had no effect on aberrant crypt foci (ACF) or colonic pH and no difference was found in oxidative changes.

Conclusions

Green coffee extract has intestinal benefits, through the action of its bioactive compounds in the microenvironment of the neoplastic lesion, thereby opening research avenues for new studies.

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绿咖啡提取物(Coffea canephora)改善了大鼠的肠道屏障，减缓了结直肠癌的进展及其相关的炎症

背景和目的癌症与炎症和氧化应激直接相关。由于其抗氧化活性，绿咖啡可能具有抗癌作用。本研究的目的是评估水溶绿咖啡提取物对结直肠癌(CRC)动物模型局部结肠形态生理和全身炎症和氧化变化的影响。方法用二甲肼诱导雄性Wistar大鼠scrc 5周。癌变10周后，将大鼠分为健康对照组(HC组，无癌诱导，无提取物组)、结直肠癌组(CRC组，有癌诱导，无提取物组)、健康绿咖啡组(HGC组，无癌诱导，有提取物组)和结直肠癌绿咖啡组(CGC组，有癌诱导，有提取物组)。使用学生t检验或方差分析和Newman-Keuls事后检验(p <0.05)。结果绿原酸和葫芦巴碱是与NF-κB相互作用的咖啡因、绿原酸和葫芦巴碱的主要来源。该提取物通过减少尿中乳果糖和甘露醇的排泄，增加粪便IgA水平，减少结肠和直肠的恶性肿瘤，有助于肠道屏障的完整性。此外，它还减少了全身炎症细胞因子TNFα和IL-6的产生。然而，绿咖啡提取物对异常隐窝灶(ACF)或结肠pH没有影响，氧化变化也没有差异。结论绿咖啡提取物通过其生物活性化合物在肿瘤病变微环境中的作用对肠道有益，从而为新的研究开辟了研究途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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