A. Mayfield, Yi-Jyun Chen, Chi-Yu Lu, Chii‐Shiarng Chen
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引用次数: 12
Abstract
Although reef-building corals are threatened by a number of anthropogenic
impacts, certain scleractinian-dinoflagellate (genus Symbiodinium) endosymbioses
have proven markedly resilient to environmental change. For instance,
corals from upwelling habitats of Southern Taiwan withstand both short- and
long-term increases in temperature, potentially due to their routine exposure
to highly variable temperature regimes in situ. To gain a greater understanding
of the proteomic basis for such acclimatization to unstable environmental
conditions, specimens of the Indo-Pacific reef-building coral Seriatopora hystrix Dana 1846 were sampled during a period of stable temperature conditions
from 1) a site characterized by frequent upwelling events in Southern
Taiwan and 2) a nearby, non-upwelling control site in the Taiwan Strait.
Two-dimensional gel electrophoresis followed by sequencing of differentially
concentrated proteins with mass spectrometry unveiled significantly more
proteins involved in the cellular stress response in coral hosts of the upwelling
site. Although such stress protein signatures could be indicative of sub-lethal
levels of cellular stress, especially given the relatively higher sediment loads
characteristic of the upwelling site, these proteins may, in contrast, have been
constitutively maintained at high levels in preparation for large fluctuations in
temperature and other abiotic parameters (e.g., nutrient levels) brought upon
by upwelling events.
虽然造礁珊瑚受到许多人为影响的威胁,但某些硬核-甲藻(共生属)内共生已被证明对环境变化具有显著的弹性。例如,来自台湾南部上升流栖息地的珊瑚可以承受短期和长期的温度升高,这可能是由于它们经常暴露在高度变化的温度环境中。为了更好地了解这种对不稳定环境条件的适应的蛋白质组学基础,在稳定的温度条件下,从1)台湾南部一个以频繁上升流事件为特征的地点和2)台湾海峡附近一个非上升流的控制地点取样了印度太平洋造礁珊瑚Seriatopora hystrix Dana 1846的标本。二维凝胶电泳和质谱测序显示,上升流区珊瑚宿主的细胞应激反应中有更多的蛋白质参与。虽然这种应激蛋白特征可能表明细胞应激的亚致死水平,特别是考虑到上升流部位相对较高的沉积物负荷特征,但相反,这些蛋白质可能在组成上保持在高水平,以应对上升流事件带来的温度和其他非生物参数(例如营养水平)的大幅波动。