Inhaled diesel exhaust alters plasma proteome signature

IF 2.7 4区医学 Q2 GENETICS & HEREDITY

Genes and Environment Pub Date : 2018-09-15 DOI:10.1183/13993003.CONGRESS-2018.PA1269

N. Mookherjee, M. Ryu, M. Hemshekhar, V. Spicer, C. Carlsten

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引用次数: 1

Abstract

Introduction: Diesel exhaust (DE), a paradigm for traffic-related air pollution, is associated with respiratory and cardiovascular diseases. The aim of this study was to define changes in global proteins (proteome) in plasma following exposure to inhaled DE, using a controlled human exposure study. Methods: Ex-smokers (n=6) inhaled filtered air (FA) and DE (300 mg PM2.5/m3) for 2h (crossover; random order). Plasma was obtained 24h after each exposure. Plasma (n=12) were probed in Slow off-rate modified aptamer (SOMAmer®)-based proteomic array. Differential analysis with Welch’s t-test was used to identify proteins significantly altered by inhaled DE compared to FA. Abundance of selected proteins were independently quantified using ELISA or immunoblots, in plasma obtained from healthy individuals following DE and FA exposure, to further validate proteins enhanced by DE. Results: 342 plasma proteins were significantly altered by DE compared to FA (Fig. 1). The top 20 proteins enhanced by DE were enriched to GO biological process of immune response; inflammation or cardiovascular disease. Conclusion: This is the first comprehensive interrogation of the plasma proteome to identify proteins altered following inhaled DE exposure in humans, and adds functional plausibility to observations of adverse health effects therein.

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吸入的柴油废气会改变血浆蛋白质组特征

导言:柴油废气(DE)是交通相关空气污染的典范，与呼吸系统和心血管疾病有关。本研究的目的是通过对照人体暴露研究，确定吸入DE后血浆中总蛋白(蛋白质组)的变化。方法:戒烟者(n=6)吸入过滤空气(FA)和DE (300 mg PM2.5/m3) 2h(交叉);随机的顺序)。每次暴露后24小时取血浆。血浆(n=12)采用基于Slow off-rate modified aptamer (SOMAmer®)的蛋白质组学阵列进行检测。采用Welch 's t检验的差异分析来鉴定吸入DE与FA相比显著改变的蛋白质。在健康个体暴露于DE和FA后获得的血浆中，使用ELISA或免疫印迹技术独立定量所选蛋白的丰度，以进一步验证DE增强的蛋白。结果:与FA相比，DE显著改变了342种血浆蛋白(图1)。被DE增强的前20种蛋白被富集为氧化石墨烯免疫反应的生物过程;炎症或心血管疾病。结论:这是第一次对血浆蛋白质组进行全面调查，以确定人类吸入DE暴露后改变的蛋白质，并为其中的不良健康影响观察增加了功能上的合理性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genes and Environment Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

4.00

自引率

0.00%

发文量

审稿时长

27 weeks

期刊介绍： Genes and Environment is an open access, peer-reviewed journal that aims to accelerate communications among global scientists working in the field of genes and environment. The journal publishes articles across a broad range of topics including environmental mutagenesis and carcinogenesis, environmental genomics and epigenetics, molecular epidemiology, genetic toxicology and regulatory sciences. Topics published in the journal include, but are not limited to, mutagenesis and anti-mutagenesis in bacteria; genotoxicity in mammalian somatic cells; genotoxicity in germ cells; replication and repair; DNA damage; metabolic activation and inactivation; water and air pollution; ROS, NO and photoactivation; pharmaceuticals and anticancer agents; radiation; endocrine disrupters; indirect mutagenesis; threshold; new techniques for environmental mutagenesis studies; DNA methylation (enzymatic); structure activity relationship; chemoprevention of cancer; regulatory science. Genetic toxicology including risk evaluation for human health, validation studies on testing methods and subjects of guidelines for regulation of chemicals are also within its scope.