Identification and characterization of a novel sesquiterpene synthase, 4-amorphen-11-ol synthase, from Artemisia maritima.

IF 1.4 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Plant Biotechnology Pub Date : 2018-06-25 DOI:10.5511/PLANTBIOTECHNOLOGY.18.0324A

Paskorn Muangphrom, H. Seki, S. Matsumoto, M. Nishiwaki, E. O. Fukushima, T. Muranaka

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引用次数: 4

Abstract

Artemisinin, a sesquiterpene lactone exhibiting effective antimalarial activity, is produced by only Artemisia annua plant. A key step in artemisinin biosynthesis is the cyclization of farnesyl pyrophosphate (FPP) to amorpha-4,11-diene catalyzed by amorpha-4,11-diene synthase (AaADS). Intriguingly, several non-artemisinin-producing Artemisia plants also express genes highly homologous to AaADS. Our previous functional analysis of these homologous enzymes revealed that they catalyzed the synthesis of rare natural sesquiterpenoids. In this study, we analyzed the function of another putative sesquiterpene synthase highly homologous to AaADS from A. maritima. Unlike AaADS, in vivo enzymatic assay showed that this enzyme cyclized FPP to 4-amorphen-11-ol, a precursor of several gastroprotective agents. The discovery of 4-amorphen-11-ol synthase (AmAOS) and the successful de novo production of 4-amorphen-11-ol in engineered yeast demonstrated herein provides insights into the methods used to enhance its production for future application.

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一种新的倍半萜合酶，4-阿莫酚-11-醇合酶的鉴定和鉴定。

青蒿素是一种倍半萜内酯，具有有效的抗疟活性，仅由青蒿属植物产生。青蒿素生物合成的一个关键步骤是在阿莫酚-4，11-二烯合酶（AaADS）的催化下，法尼焦磷酸酯（FPP）环化为阿莫酚-4,11-二烯。有趣的是，一些不产生青蒿素的青蒿素植物也表达与AaADS高度同源的基因。我们之前对这些同源酶的功能分析表明，它们催化了罕见的天然倍半萜的合成。在这项研究中，我们分析了另一种推测的倍半萜合成酶的功能，该合成酶与来自A.maritima的AaADS高度同源。与AaADS不同，体内酶测定显示，这种酶将FPP环化为4-阿莫酚-11-醇，这是几种胃保护剂的前体。本文证明的4-阿莫酚-11-醇合酶（AmAOS）的发现和在工程酵母中成功从头生产4-阿莫芬-11-醇为未来应用提供了对用于增强其生产的方法的深入了解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-PLANT SCIENCES

CiteScore

2.90

自引率

18.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Plant Biotechnology is an international, open-access, and online journal, published every three months by the Japanese Society for Plant Biotechnology. The journal, first published in 1984 as the predecessor journal, “Plant Tissue Culture Letters” and became its present form in 1997 when the society name was renamed to Japanese Society for Plant Cell and Molecular Biology, publishes findings in the areas from basic- to application research of plant biotechnology. The aim of Plant Biotechnology is to publish original and high-impact papers, in the most rapid turnaround time for reviewing, on the plant biotechnology including tissue culture, production of specialized metabolites, transgenic technology, and genome editing technology, and also on the related research fields including molecular biology, cell biology, genetics, plant breeding, plant physiology and biochemistry, metabolic engineering, synthetic biology, and bioinformatics.