Cryoprotective Effect of Pyrano-[2,3-C]-Pyrazoles on H2O2 Induced Damage in Tetrahymena thermophila

Q3 Biochemistry, Genetics and Molecular Biology

Biointerface Research in Applied Chemistry Pub Date : 2022-09-10 DOI:10.33263/briac134.305

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引用次数: 1

Abstract

Antioxidants are developed to assist the immune system and overcome oxidative stress, break the auto-oxidative chain reaction, quench oxygen singlets (O2-), and inhibit peroxidase formation. The main objective of this study was to search for potent antioxidant supplements to protect our cells against diseases associated with oxidative stress. We chose four pyrano-[2,3-c]-pyrazole derivatives to test their antioxidant properties in vivo using "Tetrahymena spp" as a cellular model. Then, we measured the activity of some antioxidant and biochemical biomarkers such as CAT, SOD, GAPDH, SDH, Gr, and MDA. Also, the fragmentation of DNA has been investigated. The current study demonstrated that the three compounds (5a, b, and c) have potent antioxidant activity, characterized by increased activity of some antioxidant enzymes, inhibiting lipid peroxidation and DNA damage. These findings suggest for the first time that Pyrano-[2,3-c]-Pyrazoles is a promising source of synthetic antioxidants that could offer protection against H2O2-induced- stress and provide us with a new challenge to design a library of pharmaceutical compounds with high activity and low toxicity.

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吡喃-[2,3-C]-吡唑类化合物对高温四膜虫H2O2损伤的低温保护作用

抗氧化剂被开发用于帮助免疫系统和克服氧化应激，打破自身氧化链式反应，猝灭氧单线态（O2-），并抑制过氧化物酶的形成。这项研究的主要目的是寻找有效的抗氧化剂补充剂，以保护我们的细胞免受与氧化应激相关的疾病的影响。我们选择了四种吡喃并[2,3-c]-吡唑衍生物，以“四膜虫spp”为细胞模型，在体内测试其抗氧化性能。然后，我们测量了一些抗氧化和生化生物标志物的活性，如CAT、SOD、GAPDH、SDH、Gr和MDA。此外，还对DNA的断裂进行了研究。目前的研究表明，这三种化合物（5a、b和c）具有强大的抗氧化活性，其特征是增加了一些抗氧化酶的活性，抑制了脂质过氧化和DNA损伤。这些发现首次表明，吡拉诺-[2,3-c]-吡唑是一种很有前途的合成抗氧化剂来源，可以提供对H2O2诱导的应激的保护，并为我们设计一个具有高活性和低毒性的药物化合物库提供了新的挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biointerface Research in Applied Chemistry CHEMISTRY, APPLIED-

CiteScore

4.80

自引率

0.00%

发文量

256

期刊介绍： Biointerface Research in Applied Chemistry is an international and interdisciplinary research journal that focuses on all aspects of nanoscience, bioscience and applied chemistry. Submissions are solicited in all topical areas, ranging from basic aspects of the science materials to practical applications of such materials. With 6 issues per year, the first one published on the 15th of February of 2011, Biointerface Research in Applied Chemistry is an open-access journal, making all research results freely available online. The aim is to publish original papers, short communications as well as review papers highlighting interdisciplinary research, the potential applications of the molecules and materials in the bio-field. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible.