In vitro examination of antioxidant and anti-cholinesterase effects of Athamanta sicula L. aqueous and methanolic extracts

Q4 Pharmacology, Toxicology and Pharmaceutics

Current Enzyme Inhibition Pub Date : 2022-07-13 DOI:10.2174/1573408017666220713100342

K. Loucif, Hassiba Benabdallah, Fatima Benchikh, C. Bensouici, Smain Amira

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Abstract

Oxidative stress plays a critical role in the pathological process of Alzheimer’s disease (AD). There is a growing interest in natural anti-oxidants and cholinesterase inhibitors from medicinal plants that may aid in the prevention against oxidative injury and in the treatment of AD. Athamanta sicula L. is an important medicinal plant. The aim of current study was to investigate the total phenolic content, antioxidant and anti-cholinesterase properties of aqueous (AqE) and methanolic extracts (MethE) from Athamanta sicula L. (A. sicula L.). The phenanthroline, ABTS free radical scavenging, β-carotene bleaching methods were utilized to assess the antioxidant capacity. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory methods were used to determine the anti-cholinesterase effect. These extracts were rich in phenolic contents. It was capable of scavenging free radicals, inhibiting lipid peroxidation, and reducing agents. A. sicula L. had a remarkable cholinesterase inhibitory effect. These results support that A. sicula L. could be a new source of anti-oxidant and anti-cholinesterase natural drugs. Further studies on the isolation and characterization of active principles of anti-oxidant and tyrosinase inhibitory activities are needed.

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柽柳水提液和甲醇提液抗氧化和抗胆碱酯酶作用的体外研究

氧化应激在阿尔茨海默病(AD)的病理过程中起着至关重要的作用。从药用植物中提取的天然抗氧化剂和胆碱酯酶抑制剂可能有助于预防氧化损伤和治疗AD，人们对它们的兴趣日益浓厚。柽柳是一种重要的药用植物。本研究主要研究了甘菊(Athamanta sicula L.)水提液(AqE)和甲醇提液(MethE)的总酚含量、抗氧化和抗胆碱酯酶活性。采用菲罗啉法、ABTS自由基清除法、β-胡萝卜素漂白法评价其抗氧化能力。采用乙酰胆碱酯酶(AChE)和丁基胆碱酯酶(BChE)抑制法测定其抗胆碱酯酶作用。这些提取物含有丰富的酚类物质。它能清除自由基，抑制脂质过氧化和还原剂。对胆碱酯酶有明显的抑制作用。这些结果支持了曲曲霉可能是抗氧化和抗胆碱酯酶天然药物的新来源。需要进一步研究其抗氧化活性和酪氨酸酶抑制活性的分离和鉴定。

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来源期刊

Current Enzyme Inhibition Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： Current Enzyme Inhibition aims to publish all the latest and outstanding developments in enzyme inhibition studies with regards to the mechanisms of inhibitory processes of enzymes, recognition of active sites, and the discovery of agonists and antagonists, leading to the design and development of new drugs of significant therapeutic value. Each issue contains a series of timely, in-depth reviews written by leaders in the field, covering a range of enzymes that can be exploited for drug development. Current Enzyme Inhibition is an essential journal for every pharmaceutical and medicinal chemist who wishes to have up-to-date knowledge about each and every development in the study of enzyme inhibition.