Proteomics analysis of carcinogenesis in a rat model of mammary cancer induced by DMBA (7,12-dimethylbenz[a]anthracene).

Q2 Pharmacology, Toxicology and Pharmaceutics

F1000Research Pub Date : 2024-08-05 eCollection Date: 2023-01-01 DOI:10.12688/f1000research.132524.1

Dyah Ayu Oktavianie Ardhiana Pratama, Anggun Nur Cahyati, Ulayatul Kustiati, Andreas Bandang Hardian, Fajar Shodiq Permata

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引用次数: 0

Abstract

Background: Mammary cancer, called breast cancer in humans, results from the abnormal growth of cells in the mammary glands that attack the surrounding tissue. The process of carcinogenesis at the molecular level can be monitored through the production of proteins as biomarkers for carcinogenesis. 7,12-Dimethylbenz[a]anthracene (DMBA) is a known carcinogenic compound. This study aimed to analyze the proteomic profile as critical data regarding DMBA-induced carcinogenesis in Sprague‒Dawley rats. Methods: Experimental animals were divided into two groups: a treatment group given DMBA at a dose of 10 mg/kg (intramammary) at intervals of 48 hours for a total of 10 doses, and a negative control group that was not given any treatment. Measurement of the total protein concentration was carried out using a spectrophotometer, and the data were analyzed using a t-test, while the characterization of protein profiles was carried out based on molecular weight data using SDS‒PAGE. Mammary gland histopathology was evaluated by hematoxylin and eosin (H&E) staining. Results: The results showed a significant (p<0.05) increase of 27% in the total protein concentration in the rat mammary cancer model. The results of proteomic characterization showed a protein profile containing proteins of 187, 169, 68, 64, 53, 41, 24, 18, and 14 kDa, which were suspected to be HER-2, Nischarin, COX-2, Albumine, Vimentin, ACTB, TNF, p16, and fatty acid binding protein 3 (FABP3), respectively. Histopathology of the mammary glands showed an irregular and indistinct arrangement of the alveoli and extensive epithelial cell proliferation from the surface to the lumen of the mammary ducts, and the mammary stroma showed the formation of new epithelial cells, which were cancer cells that spread to surrounding tissue. Conclusions: The proteomic profile was strongly associated with morphological alterations in mammary carcinogenesis in a rat model of DMBA-induced mammary cancer.

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DMBA(7,12-二甲基苯[a]蒽)致大鼠乳腺癌模型癌变的蛋白质组学分析

背景：乳腺癌症，在人类中被称为癌症，是由乳腺中攻击周围组织的细胞异常生长引起的。可以通过产生作为致癌生物标志物的蛋白质来监测分子水平上的致癌过程。7,12-二甲基苯并[a]蒽（DMBA）是一种已知的致癌化合物。本研究旨在分析蛋白质组学图谱，作为DMBA诱导Sprague-Dawley大鼠致癌的关键数据。方法：实验动物分为两组：治疗组给予DMBA，剂量为10mg/kg（肌内），间隔48小时，共10剂，阴性对照组不给予任何治疗。使用分光光度计测量总蛋白质浓度，并使用t检验分析数据，同时使用SDS-PAGE基于分子量数据对蛋白质图谱进行表征。苏木精和伊红（H&E）染色评估乳腺组织病理学。结果：大鼠乳腺癌症模型总蛋白浓度显著升高27%（p＜0.05）。蛋白质组学表征的结果显示蛋白质谱包含187、169、68、64、53、41、24、18和14kDa的蛋白质，这些蛋白质分别被怀疑是HER-2、尼沙林、COX-2、白蛋白、波形蛋白、ACTB、TNF、p16和脂肪酸结合蛋白3（FABP3）。乳腺的组织病理学显示肺泡排列不规则且模糊，上皮细胞从乳腺导管表面到管腔广泛增殖，乳腺间质显示新上皮细胞的形成，这些细胞是扩散到周围组织的癌症细胞。结论：在DMBA诱导的癌症大鼠模型中，蛋白质组学图谱与乳腺癌发生的形态学改变密切相关。

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来源期刊

F1000Research Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

5.00

自引率

0.00%

发文量

1646

审稿时长

1 weeks

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