Characterization and comparison of lipids in Camellia oleifera kernels of XL210 and XL1 based on LC-MS/MS

Reproduction and breeding Pub Date : 2021-12-01 DOI:10.1016/j.repbre.2021.12.003

Hai Xie , Fenglin Chen , Huangping Yin, Guo Peng, Changqiao You, Pai Qin, Shuai Jiang, Xinhong Guo

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引用次数: 2

Abstract

Camellia oleifera is a unique edible oil tree species in China and one of the four major woody oil tree species in the world. Its products are widely used in forestry, agriculture and fishery, medicine, daily chemicals, light textile and so on. In recent years, C. oleifera has become a topic of great scientific interest because of its unique value. However, few lipidomics studies on C. oleifera were reported. In this study, “XiangLin1” (XL1) and “XiangLin210” (XL210) were analyzed by non-targeted LC-MS/MS and 1329 metabolites were identified. The 99 significantly different lipids (SDLs) were matched with the criteria of P < 0.05 and variable importance in the projection (VIP) value > 1. Compared with XL1, XL210 has 59 SDLs down-regulated and 40 SDLs up-regulated. Through correlation analysis, we concluded that glycerolipids (GLs), glycerophospholipids (GPs) and sphingolipids (SPs) had strong correlation within and between classes. Our results will provide a reference for the subsequent study on the lipid synthesis mechanism of C. oleifera by the multi-omics technology.

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基于LC-MS/MS的XL210和XL1油茶籽粒脂质特征及比较

油茶(Camellia oleifera)是中国特有的食用油树，也是世界四大木本油树之一。产品广泛应用于林业、农渔业、医药、日化、轻纺等行业。近年来，油桐因其独特的价值而成为科学研究的热点。然而，关于油桐脂质组学的研究报道较少。本研究对“XiangLin1”(XL1)和“XiangLin210”(XL210)进行了非靶向LC-MS/MS分析，鉴定出1329种代谢物。99个显著不同的脂质(SDLs)符合P <标准;0.05和变量重要性在投影(VIP)值>1。与XL1相比，XL210有59个SDLs下调，40个SDLs上调。通过相关分析，我们发现甘油脂(GLs)、甘油磷脂(GPs)和鞘脂(SPs)在类内和类间具有很强的相关性。本研究结果将为后续利用多组学技术研究油葵脂质合成机制提供参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproduction and breeding

CiteScore

2.40

自引率

0.00%

发文量