Identification for metabolism profiles and pharmacokinetic studies of tradition Chinese prescription Ji-Ming-San and its major metabolites in rats by UHPLC-Q-TOF-MS/MS and UHPLC-MS/MS

IF 2.6 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Food and Drug Analysis Pub Date : 2023-08-31 DOI:10.38212/2224-6614.3473

Cheng-Yang Hsieh, Ching-Chiung Wang, L. Tayo, Po-Wei Tsai, Chia-Jung Lee

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引用次数: 1

Abstract

Ji-Ming-Shan (JMS) is a traditional prescription use for patients with rheumatism, tendons swelling, athlete ' s foot, diuresis and even gout. This study developed a rapid and sensitive method for the analysis of JMS chemical components in the Traditional Chinese medicine (TCM) prescription and in the serum samples of rats which were administered with the herbal extract. Two mass spectrometric approaches were used namely Ultra-performance liquid chromatography-quadrupole time-of-ﬂ ight-mass spectrometry (UPLC-Q-TOF-MS) method for the major metabolites of the JMS extract while Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was employed for the detection of the JMS metabolites in the sera of rats. It was revealed that the major components in the JMS extract were identi ﬁ ed to be narirutin and hesperidin. It was con ﬁ rmed that 17 compounds were determined in JMS prescription extract and 16 metabolites resulting from the biotransformation of narirutin and hesperidin were identi ﬁ ed in the serum samples. In silico analyses also revealed that the metabolite hersperidin-7-glucoside exhibited the best binding ability with respect to the Cyclooxygenase-2 (COX-2) enzyme target. This study showcased the possible biochemical mechanism involved in the therapeutic ef ﬁ ciency of JMS components and their biotransformation products.

查看原文本刊更多论文

采用UHPLC-Q-TOF-MS/MS和UHPLC-MS/MS对中药复方吉明散及其主要代谢物在大鼠体内的代谢谱及药动学进行鉴定

积明山是治疗风湿病、肌腱肿胀、脚气、利尿甚至痛风的传统方剂。本研究建立了一种快速、灵敏的方法来分析中药处方和大鼠血清样品中JMS的化学成分。采用超高效液相色谱-四极杆飞行时间质谱法(UPLC-Q-TOF-MS)对大鼠血清中JMS主要代谢物进行检测，采用超高效液相色谱-串联质谱法(UPLC-MS/MS)对大鼠血清中JMS代谢物进行检测。结果表明，JMS提取物的主要成分为丁香苷和橙皮苷。从JMS方提取物中鉴定出17个化合物，从血清样品中鉴定出narirutin和橙皮苷生物转化产生的16种代谢物。硅分析还表明，其代谢物hersperidin-7-glucoside与环氧合酶-2 (COX-2)酶靶点的结合能力最好。本研究揭示了JMS组分及其生物转化产物治疗效果的可能生化机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Food and Drug Analysis 医学-食品科技

CiteScore

6.30

自引率

2.80%

发文量

审稿时长

3.8 months

期刊介绍： The journal aims to provide an international platform for scientists, researchers and academicians to promote, share and discuss new findings, current issues, and developments in the different areas of food and drug analysis. The scope of the Journal includes analytical methodologies and biological activities in relation to food, drugs, cosmetics and traditional Chinese medicine, as well as related disciplines of topical interest to public health professionals.