Sensing a SARS-CoV-2 spike peptide using a titanium carbide-doped imprinted polymer-coated extended-gate field effect transistor

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research Pub Date : 2023-08-01 DOI:10.1016/j.sbsr.2023.100577

Mei-Hwa Lee , Cheng-Chih Lin , James L. Thomas , Chen-Yuan Chen , Chuen-Yau Chen , Chien-Hsin Yang , Hung-Yin Lin

{"title":"Sensing a SARS-CoV-2 spike peptide using a titanium carbide-doped imprinted polymer-coated extended-gate field effect transistor","authors":"Mei-Hwa Lee , Cheng-Chih Lin , James L. Thomas , Chen-Yuan Chen , Chuen-Yau Chen , Chien-Hsin Yang , Hung-Yin Lin","doi":"10.1016/j.sbsr.2023.100577","DOIUrl":null,"url":null,"abstract":"<div>The COVID-19 pandemic of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has over 750 million confirmed cases globally and more than six million deaths. Several variants have been named and identified as variants of concern by the World Health Organization (WHO); these include the Delta and Omicron variants. This work demonstrates the integration of epitope-imprinted conductive polymers with extended gate field effect transistors for the sensitive detection of the covid spike protein. Peptides from the receptor-binding domain on the spike protein were synthesized and imprinted onto poly(aniline-co-3-aminobenzenesulfonic acid), poly(AN-co-MSAN), by electropolymerization. Doping the conductive polymer film with titanium carbide (Ti2C) strengthened the electrochemical response approximately 1.5-fold. The FET platform not only amplified the electrochemical response about two-fold (compared with electrode-based sensing), but also lowered the sensing range for the SARS-CoV-2 spike protein subunit S1 (ncovS1) from 1.0 to 0.01 fg/mL.</div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"41 ","pages":"Article 100577"},"PeriodicalIF":5.4000,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214180423000296/pdfft?md5=0c995ff16ea753dc6f0703303e3f9f85&pid=1-s2.0-S2214180423000296-main.pdf","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensing and Bio-Sensing Research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214180423000296","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 1

Abstract

The COVID-19 pandemic of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has over 750 million confirmed cases globally and more than six million deaths. Several variants have been named and identified as variants of concern by the World Health Organization (WHO); these include the Delta and Omicron variants. This work demonstrates the integration of epitope-imprinted conductive polymers with extended gate field effect transistors for the sensitive detection of the covid spike protein. Peptides from the receptor-binding domain on the spike protein were synthesized and imprinted onto poly(aniline-co-3-aminobenzenesulfonic acid), poly(AN-co-MSAN), by electropolymerization. Doping the conductive polymer film with titanium carbide (Ti₂C) strengthened the electrochemical response approximately 1.5-fold. The FET platform not only amplified the electrochemical response about two-fold (compared with electrode-based sensing), but also lowered the sensing range for the SARS-CoV-2 spike protein subunit S1 (ncovS1) from 1.0 to 0.01 fg/mL.

查看原文本刊更多论文

利用碳化钛掺杂的印迹聚合物包覆扩展栅场效应晶体管检测SARS-CoV-2尖峰肽

COVID-19大流行的严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)全球确诊病例超过7.5亿例，死亡人数超过600万。世界卫生组织(世卫组织)已将若干变体命名并确定为令人关注的变体;其中包括Delta和Omicron变体。这项工作证明了表位印迹导电聚合物与扩展门场效应晶体管的集成，可用于灵敏检测covid刺突蛋白。从刺突蛋白上的受体结合区域合成多肽，并通过电聚合将其印迹到聚(苯胺-co-3-氨基苯磺酸)聚(AN-co-MSAN)上。在导电聚合物薄膜中掺杂碳化钛(Ti2C)，其电化学响应增强了约1.5倍。FET平台不仅将电化学响应放大了两倍(与电极传感相比)，而且将SARS-CoV-2刺突蛋白亚基S1 (ncovS1)的传感范围从1.0 fg/mL降低到0.01 fg/mL。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Sensing and Bio-Sensing Research Engineering-Electrical and Electronic Engineering

CiteScore

10.70

自引率

3.80%

发文量

审稿时长

87 days

期刊介绍： Sensing and Bio-Sensing Research is an open access journal dedicated to the research, design, development, and application of bio-sensing and sensing technologies. The editors will accept research papers, reviews, field trials, and validation studies that are of significant relevance. These submissions should describe new concepts, enhance understanding of the field, or offer insights into the practical application, manufacturing, and commercialization of bio-sensing and sensing technologies. The journal covers a wide range of topics, including sensing principles and mechanisms, new materials development for transducers and recognition components, fabrication technology, and various types of sensors such as optical, electrochemical, mass-sensitive, gas, biosensors, and more. It also includes environmental, process control, and biomedical applications, signal processing, chemometrics, optoelectronic, mechanical, thermal, and magnetic sensors, as well as interface electronics. Additionally, it covers sensor systems and applications, µTAS (Micro Total Analysis Systems), development of solid-state devices for transducing physical signals, and analytical devices incorporating biological materials.