Chemoenzymatic synthesis of 6′-sialolactose-modified nanobody

IF 1.2 4区化学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Carbohydrate Chemistry Pub Date : 2022-01-01 DOI:10.1080/07328303.2022.2055048

Min Jiang , Jie Zhao , Guodong Mei , Han Lin , Haofei Hong , Dan Li , Zhimeng Wu

引用次数: 1

Abstract

6′-Sialolactose-modified mono- and bivalent nanobodies were designed and synthesized by a chemoenzymatic strategy. Epidermal growth factor receptor (EGFR)-targeting 7D12 nanobodies, including mono- and bivalent 7D12, were recombinantly expressed in Escherichia coli. Triglycine-modified 6′-sialolactose derivative was synthesized in nine steps. The conjugation of carbohydrates with nanobodies was achieved by Sortase-A mediated ligation with high conversion rates. The obtained glycoengineered nanobodies were finally verified by SDS-PAGE, western blot, and mass spectrometry.

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6′-唾液乳糖修饰纳米体的化学酶合成

采用化学酶法设计和合成了6′-唾液乳糖修饰的单价和二价纳米体。在大肠杆菌中重组表达了表皮生长因子受体(EGFR)靶向7D12纳米体，包括单价和二价7D12。通过9步合成了甘油三酯修饰的6′-唾液乳糖衍生物。碳水化合物与纳米体的结合是通过Sortase-A介导的高转化率连接实现的。获得的糖工程纳米体最终通过SDS-PAGE、western blot和质谱进行验证。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Carbohydrate Chemistry 化学-生化与分子生物学

CiteScore

2.10

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Carbohydrate Chemistry serves as an international forum for research advances involving the chemistry and biology of carbohydrates. The following aspects are considered to fall within the scope of this journal: -novel synthetic methods involving carbohydrates, oligosaccharides, and glycoconjugates- the use of chemical methods to address aspects of glycobiology- spectroscopic and crystallographic structure studies of carbohydrates- computational and molecular modeling studies- physicochemical studies involving carbohydrates and the chemistry and biochemistry of carbohydrate polymers.