The Relation Between Host TLR9 -1486T/C, Rs187084 Gene Polymorphisms and Helicobacter Pylori CagA, sodB, hsp60, and vacA Virulence Genes Among Gastric Cancer Patients

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2022-02-27 DOI:10.33073/pjm-2022-003

A. Sultan, R. Shenouda, A. Sultan, A. Shehta, Y. Nabiel

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引用次数: 4

Abstract

Abstract To identify the associations between different genotypes of TLR9 -1486T/C (rs187084) with gastric cancer patients and reveal their relation to Helicobacter pylori virulence genes (cagA, sodB, hsp60 and vacA). Patients with gastric cancer were recruited to our study, diagnosed both endoscopically and histopathologically. H. pylori were isolated from gastric samples by culture and PCR amplification of the glmM gene. Virulence genes cagA, sodB, hsp60, and vacA were detected by multiplex PCR. Blood samples were used for genotyping of TLR9 -1486T/C (rs187084) by PCR-RFLP. Out of 132 patients with gastric cancer, 106 (80.3%) were positive for H. pylori. A similar number of healthy participants was recruited as controls. The prevalence of cagA, sodB, hsp60, and vacA genes among H. pylori was 90.6%, 70.8%, 83.0%, and 95.3%, respectively. The vacA gene alleles had a prevalence of 95.3% for vacAs1/s2, 52.8% for vacAm1, and 42.5% for vacAm2. The CC genotype of TLR9 -1486T/C had a significantly higher frequency in gastric cancer patients when compared to healthy participants (p = 0.045). Furthermore, the CC genotype demonstrated a significant association with H. pylori strains carrying sodB, hsp60, and vacAm1 virulence genes (p = 0.021, p = 0.049, and p = 0.048 respectively). Patients with CC genotype of TLR9 -1486T/C (rs187084) might be at higher risk for the development of gastric cancer, and its co-existence with H. pylori strains carrying sodB, hsp60, or vacAm1 virulence genes might have a synergistic effect in the development of gastric cancer. Further studies on a wider scale are recommended.

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胃癌患者宿主TLR9 -1486T/C、Rs187084基因多态性与幽门螺杆菌CagA、sodB、hsp60、vacA毒力基因的关系

摘要探讨不同基因型TLR9-1486T/C（rs187084）与癌症患者的关系，揭示其与幽门螺杆菌毒力基因（cagA、sodB、hsp60和vacA）的关系。癌症患者被纳入我们的研究，经内镜和组织病理学诊断。通过培养和扩增glmM基因从胃样品中分离幽门螺杆菌。采用多重聚合酶链式反应检测病毒基因cagA、sodB、hsp60和vacA。采用PCR-RFLP方法对TLR9-1486T/C（rs187084）进行基因分型。在132例癌症患者中，106例（80.3%）幽门螺杆菌阳性。同样数量的健康参与者被招募作为对照。幽门螺杆菌中cagA、sodB、hsp60和vacA基因的患病率分别为90.6%、70.8%、83.0%和95.3%。vacAs1/s2的vacA基因等位基因的患病率为95.3%，vacAm1为52.8%，vacAm2为42.5%。与健康参与者相比，TLR9-1486T/C的CC基因型在癌症患者中的频率显著更高（p=0.045）。此外，CC基因型与携带sodB、hsp60和vacAm1毒力基因的幽门螺杆菌菌株显著相关（分别为p=0.021、p=0.049和p=0.048）。具有TLR9-1486T/C（rs187084）CC基因型的患者可能具有更高的胃癌发展风险，其与携带sodB、hsp60或vacAm1毒力基因的幽门螺杆菌菌株共存可能在癌症的发展中具有协同作用。建议进行更大规模的进一步研究。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464