A Composite of Hepatocyte Growth Factor- and 5α-Dihydrotestosterone-Gelatin Microspheres with Adipose-Derived Stem Cells Enhances Wound Healing

IF 2.8 4区医学 Q2 DERMATOLOGY

Skin Pharmacology and Physiology Pub Date : 2022-04-19 DOI:10.1159/000524188

K. Tao, X. Bai, P. Ji, Yue Zhang, Tao Cao, F. Han, Zhi Zhang, Hao Guan, D. Hu

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引用次数: 1

Abstract

Introduction: Reconstructing sebaceous glands is one goal of functionally healing patients who have suffered severe burns, instead of the simple pursuit of wound closure. Effective regeneration of skin appendages remains a challenge in skin wound management and research. Objective: The aim of this study was to evaluate the differentiation of adipose-derived stem cells (ADSCs) into sebaceous glands and clarified the involvement of hepatocyte growth factor (HGF) and 5α-dihydrotestosterone (5α-DHT) in this process. Methods: This study used HGF- and 5α-DHT-gelatin microspheres to treat human ADSCs and investigated the reconstruction of sebaceous glands. HGF- and 5α-DHT-gelatin microspheres were constructed using microcapsule slow-release technology. A mice full-thickness skin-wound model was established to evaluate wound healing, and hematoxylin-eosin staining was utilized to determine the skin structure. Results: In vitro analyses found that HGF- and 5α-DHT-gelatin microspheres promoted migration of and tube formation by ADSCs. Furthermore, AKT/ERK signaling, which is related to sebocyte and sweat gland epithelial-cell growth, was activated after HGF and 5α-DHT treatment. An in vivo wound healing model demonstrated that ADSCs primed with amnion-loaded HGF- and 5α-DHT-gelatin microspheres promoted wound healing and increased sebaceous gland formation compared to the control group. Conclusions: This study confirms the efficacy of ADSCs treated with amnion and HGF- and 5α-DHT-gelatin microspheres in accelerating wound healing and effectively restoring sebaceous glands. This engineered tissue provides insight into and a novel therapeutic material for burns and full-thickness skin wounds.

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肝细胞生长因子-和5α-二氢睾酮明胶微球与脂肪来源干细胞的复合物增强伤口愈合

简介：重建皮脂腺是功能性治愈严重烧伤患者的目标之一，而不是简单地追求伤口闭合。皮肤附属物的有效再生仍然是皮肤伤口管理和研究中的一个挑战。目的：本研究旨在评估脂肪干细胞（ADSCs）向皮脂腺的分化，并阐明肝细胞生长因子（HGF）和5α-二氢睾酮（5α-DHT）在这一过程中的作用。方法：采用HGF-和5α-DHT明胶微球治疗人ADSCs，并研究其对皮脂腺的重建作用。采用微胶囊缓释技术构建了HGF-和5α-DHT明胶微球。建立小鼠全层皮肤伤口模型以评估伤口愈合，并使用苏木精-伊红染色来确定皮肤结构。结果：体外分析发现，HGF-和5α-DHT明胶微球促进了ADSCs的迁移和成管。此外，与皮脂细胞和汗腺上皮细胞生长有关的AKT/ERK信号传导在HGF和5α-DHT处理后被激活。体内伤口愈合模型表明，与对照组相比，用羊膜负载的HGF-和5α-DHT明胶微球引发的ADSCs促进了伤口愈合并增加了皮脂腺的形成。结论：本研究证实了羊膜和HGF-和5α-DHT明胶微球治疗ADSCs在加速伤口愈合和有效修复皮脂腺方面的疗效。这种工程组织为烧伤和全层皮肤伤口提供了深入了解和新型治疗材料。

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来源期刊

Skin Pharmacology and Physiology 医学-皮肤病学

CiteScore

5.20

自引率

7.40%

发文量

审稿时长

>12 weeks

期刊介绍： In the past decade research into skin pharmacology has rapidly developed with new and promising drugs and therapeutic concepts being introduced regularly. Recently, the use of nanoparticles for drug delivery in dermatology and cosmetology has become a topic of intensive research, yielding remarkable and in part surprising results. Another topic of current research is the use of tissue tolerable plasma in wound treatment. Stimulating not only wound healing processes but also the penetration of topically applied substances into the skin, this novel technique is expected to deliver very interesting results.