AEDG Peptide Prevents Oxidative Stress in the Model of Induced Aging of Skin Fibroblasts

IF 0.6 Q4 GERIATRICS & GERONTOLOGY

Advances in Gerontology Pub Date : 2022-07-12 DOI:10.1134/S2079057022020096

E. O. Gutop, N. S. Linkova, E. O. Kozhevnikova, N. V. Fridman, O. M. Ivko, V. Kh. Khavinson

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Abstract

Ultraviolet (UV) radiation is one of the main environmental factors leading to oxidative stress and accelerated skin aging. AEDG peptide, a regulator of pineal gland functions, has demonstrated geroprotective and antioxidant effects in in vivo and in vitro studies. The aim of this research was to evaluate the effect of AEDG peptide on the expression of genes encoding enzymes of the antioxidant system (NQO1, SOD1, CATALASE, and TRXR) in human skin fibroblasts in a model of accelerated aging induced by UV radiation. The expression of SOD1 and TXNRD1 genes during UV-induced aging of dermal fibroblasts increases by 2 and 1.7 times, respectively. This can be considered as a defense mechanism against oxidative stress caused by UV radiation. Photoaging does not influence the expression of the NQO1 and CATALASE genes in the culture of skin fibroblasts. AEDG peptide promotes the expression of the SOD-1, NQO1, and CATALASE genes in dermal fibroblasts exposed to UV radiation by 2.7, 2.6, and 3.2 times, respectively. AEDG peptide can stimulate the expression of these genes via the Keap1/Nrf2 signaling pathway. AEDG peptide might be potentially effective in preventing the accelerated aging of dermal fibroblasts.

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AEDG肽对皮肤成纤维细胞诱导衰老模型氧化应激的抑制作用

紫外线（UV）辐射是导致氧化应激和加速皮肤衰老的主要环境因素之一。AEDG肽是松果体功能的调节因子，在体内外研究中已显示出促性腺激素保护和抗氧化作用。本研究的目的是评估AEDG肽对紫外线辐射诱导的加速衰老模型中人类皮肤成纤维细胞中编码抗氧化系统酶（NQO1、SOD1、CATLASE和TRXR）的基因表达的影响。SOD1和TXNRD1基因在紫外线诱导的真皮成纤维细胞衰老过程中的表达分别增加了2倍和1.7倍。这可以被认为是对紫外线辐射引起的氧化应激的防御机制。光老化不影响皮肤成纤维细胞培养中NQO1和CATLASE基因的表达。AEDG肽在暴露于紫外线辐射的真皮成纤维细胞中分别促进SOD-1、NQO1和CATLASE基因的表达2.7倍、2.6倍和3.2倍。AEDG肽可以通过Keap1/Nrf2信号通路刺激这些基因的表达。AEDG肽可能在预防真皮成纤维细胞加速老化方面具有潜在的有效性。

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来源期刊

Advances in Gerontology GERIATRICS & GERONTOLOGY-

CiteScore

0.80

自引率

16.70%

发文量

期刊介绍： Advances in Gerontology focuses on biomedical aspects of aging. The journal also publishes original articles and reviews on progress in the following research areas: demography of aging; molecular and physiological mechanisms of aging, clinical gerontology and geriatrics, prevention of premature aging, medicosocial aspects of gerontology, and behavior and psychology of the elderly.