Xiaolan Li, Huan Hu, Qunli Ren, Miao Wang, Yimei Du, Yuqi He, Qian Wang
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引用次数: 0
Abstract
Dendrobium officinale usually lives on rock or tree, but their endophyte diversity has not yet been fully revealed? In this study, high-throughput sequencing technology was used to investigate the endophyte diversity of the roots of D. officinale lived on tree (Group 1-3, arboreal type) and rock (Group 4, lithophytic type). The results showed that their composition of endophytic fungi and bacteria were similar at phylum level, while their relative abundance were different. Their taxa composition and abundance of endophytes differed significantly among groups at the genus level. Alpha diversity of endophytic fungi of lithophytic type was higher than those from arboreal type, while there was no advantage in endophytic bacteria. Beta diversity revealed that the endophytic fungi tended to cluster in each group, but the endophytic bacteria were dispersed among the groups. LEfSe analysis found that the numbers of predicted endophyte biomarkers of lithophytic type were more than arboreal types at genus level, and the biomarkers varied among groups. Microbial network analysis revealed similarities and differences in the taxa composition and abundance of shared and special endophytes in each group. These results suggested that the root endophytes of lithophytic and arboreal D. officinale differed in diversity.
期刊介绍:
Plant Biotechnology is an international, open-access, and online journal, published every three months by the Japanese Society for Plant Biotechnology. The journal, first published in 1984 as the predecessor journal, “Plant Tissue Culture Letters” and became its present form in 1997 when the society name was renamed to Japanese Society for Plant Cell and Molecular Biology, publishes findings in the areas from basic- to application research of plant biotechnology. The aim of Plant Biotechnology is to publish original and high-impact papers, in the most rapid turnaround time for reviewing, on the plant biotechnology including tissue culture, production of specialized metabolites, transgenic technology, and genome editing technology, and also on the related research fields including molecular biology, cell biology, genetics, plant breeding, plant physiology and biochemistry, metabolic engineering, synthetic biology, and bioinformatics.