Vitamin E ameliorates disturbances in testosterone pathway and sperm quality of male rats induced by the glycosides vicine and convicine of Vicia faba

IF 1 Q4 FOOD SCIENCE & TECHNOLOGY

Nutrition & Food Science Pub Date : 2021-08-03 DOI:10.1108/nfs-02-2021-0053

K. Koriem, M. Arbid

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Abstract

Purpose This paper aims to design to evaluate the protective effect of vitamin E to ameliorate the disturbances in testosterone pathway and sperm quality of male rats induced by the glycosides vicine (V) and convicine (C) of Vicia faba. Design/methodology/approach Forty male albino rats were divided into five equal groups; control, paraffin oil, V (400 mg/kg) C (150 mg/kg)-treated group, vitamin E (100 mg/kg) + VC-treated group, and vitamin E (200 mg/kg) + VC-treated groups which injected intraperioneally (IP) with 0.5-ml saline, 0.5-ml paraffin oil,V (400 mg/kg) and C (150 mg/kg) of Vicia faba, vitamin E (100 mg/kg) + VC-treated groups, and Vitamin E(200 mg/kg) + VC-treated groups, respectively. Blood and testicular tissue were obtained after one month of the study. The male genital organs were calculated. Testosterone (Ts), luteinizing hormone (LH), follicle stimulating hormone (FSH), dehydroepiandrosterone sulfate (DHEA-SO4), sex hormone binding globulin (SHBG),?-glutamyl transpeptidase (?-GT), glucose-6-phosphate dehydrogenase (G6PD), 3ß-hydroxysteroid dehydrogenase (3ßHSD), lactate dehydrogenase (LDH), spermatozoa concentration, percent of mortality and abnormal sperms were evaluated. Findings The VC-treated group showed significant decrease (p < 0.01) in Ts, DHEA-SO4, G6PD, spermatozoa number and mortality percent, as well as, the male genital organs (testes, epidydemis, seminal vesicle, prostate and vasa deferentia) while significant increase (p < 0.01) was found in LH, FSH, SHBG, LDH, ?-GT, sperms monoclonal Ki-67, and abnormal spermatocytes levels compared with control group. Vitamin E co-injection with VC-treated group returned all these parameters to the normal values. The higher dose of vitamin E (200 mg/kg) was more effect than the lower dose (100 mg/kg). Originality/value Vicia faba contains V and C glycosides. The V and C glycosides in Vicia faba are hydrolyzed by intestinal microflora to aglycones divicine and isouramil, respectively. Divicine and isouramil are highly reactive compounds generating free radicals where divicine and isouramil are the main factors of favism. The V and C glycosides induced disturbances in testosterone pathway and sperm quality of male rats and vitamin E ameliorates these disturbances.

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维生素E可改善蚕豆苷类药物对雄性大鼠睾丸激素通路和精子质量的影响

目的探讨维生素E对蚕豆苷类药物vicine (V)和vicine (C)所致雄性大鼠睾丸激素通路和精子质量紊乱的保护作用。设计/方法/方法40只雄性白化大鼠分为5组;对照组、石蜡油、V (400 mg/kg) C (150 mg/kg)处理组、维生素E(100 mg/kg) + vc处理组、维生素E(200 mg/kg) + vc处理组，分别腹腔注射0.5 ml生理盐水、0.5 ml石蜡油、蚕豆V (400 mg/kg)和C (150 mg/kg)、维生素E(100 mg/kg) + vc处理组和维生素E(200 mg/kg) + vc处理组。研究一个月后采集血液和睾丸组织。计算男性生殖器官。睾酮(Ts)、促黄体生成素(LH)、促卵泡激素(FSH)、硫酸脱氢表雄酮(DHEA-SO4)、性激素结合球蛋白(SHBG)、?评估-谷氨酰转肽酶(?-GT)、葡萄糖-6-磷酸脱氢酶(G6PD)、3ß-羟基类固醇脱氢酶(3ßHSD)、乳酸脱氢酶(LDH)、精子浓度、死亡率和异常精子率。结果vc治疗组Ts、DHEA-SO4、G6PD、精子数量、死亡率及男性生殖器官(睾丸、附睾、精囊、前列腺、输精管)水平显著低于对照组(p < 0.01)， LH、FSH、SHBG、LDH、γ -GT、精子单克隆Ki-67、异常精母细胞水平显著高于对照组(p < 0.01)。维生素E联合vc处理组各项指标均恢复正常。高剂量维生素E (200 mg/kg)比低剂量(100 mg/kg)效果更好。蚕豆含有V和C糖苷。蚕豆中的V和C糖苷分别被肠道菌群水解为divicine苷元和异脲胺苷元。丁嘌呤和异脲胺是产生自由基的高活性化合物，其中丁嘌呤和异脲胺是导致肥胖的主要因素。V和C糖苷引起雄性大鼠睾酮通路和精子质量紊乱，维生素E可改善这些紊乱。

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来源期刊

Nutrition & Food Science FOOD SCIENCE & TECHNOLOGY-

CiteScore

2.80

自引率

8.30%

发文量

期刊介绍： Nutrition & Food Science* (NFS) is an international, double blind peer-reviewed journal offering accessible and comprehensive coverage of food, beverage and nutrition research. The journal draws out the practical and social applications of research, demonstrates best practice through applied research and case studies and showcases innovative or controversial practices and points of view. The journal is an invaluable resource to inform individuals, organisations and the public on modern thinking, research and attitudes to food science and nutrition. NFS welcomes empirical and applied research, viewpoint papers, conceptual and technical papers, case studies, meta-analysis studies, literature reviews and general reviews which take a scientific approach to the following topics: -Attitudes to food and nutrition -Healthy eating/ nutritional public health initiatives, policies and legislation -Clinical and community nutrition and health (including public health and multiple or complex co-morbidities) -Nutrition in different cultural and ethnic groups -Nutrition during pregnancy, lactation, childhood, and young adult years -Nutrition for adults and older people -Nutrition in the workplace -Nutrition in lower and middle income countries (incl. comparisons with higher income countries) -Food science and technology, including food processing and microbiological quality -Genetically engineered foods -Food safety / quality, including chemical, physical and microbiological analysis of how these aspects effect health or nutritional quality of foodstuffs