Resonance assignments of the ORC2-WH domain of the human ORC protein

IF 0.8 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2022-08-11 DOI:10.1007/s12104-022-10100-8

Lanlan Song, Xiaoming Tu, Jiahai Zhang

引用次数: 0

Abstract

ORC2 is a small subunit of the origin recognition complex (ORC), which is important for gene replication. The ORC2 WH domain recognizes dsDNA sequences with its flexible β-sheet hairpins as anchors. Here, we report near-complete NMR backbone and side chain resonance assignments of the WH domain and study the backbone relaxation of the WH domain. These studies will contribute to further understanding of the structure–function relationship of the ORC protein.

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人ORC蛋白ORC2-WH结构域的共振配位

ORC2是起源识别复合体(origin recognition complex, ORC)的一个小亚基，在基因复制中起重要作用。ORC2 WH结构域以其灵活的β片发夹作为锚点识别dsDNA序列。在这里，我们报告了WH结构域的近完整的核磁共振主链和侧链共振分配，并研究了WH结构域的主链弛豫。这些研究将有助于进一步了解ORC蛋白的结构-功能关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.