Preparation of CaO-SiO2-CuO bioactive glasses-embedded anodic alumina with improved biological activities

Q1 Materials Science

Biomedical Glasses Pub Date : 2017-10-26 DOI:10.1515/bglass-2017-0006

Si-yu Ni, Lin Mei, Shirong Ni, Ran Cui, Xiaohong Li, Feng F Hong, T. Webster, Chengtie Wu

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引用次数: 1

Abstract

Abstract To improve bone cell cytocompatibility properties of porous anodic alumina (PAA) and implement anti-bacterial properties, amorphous CaO-SiO2-CuO materials were loaded into PAA nano-pores (termed CaO-SiO2- CuO/PAA) by a facile ultrasonic-assisted sol-dipping strategy. The surface features and chemistry of the obtained CaO-SiO2-CuO/PAA were investigated by a field emission scanning microscope (FESEM), an energy-dispersive Xray spectrometer (EDS) and an X-ray photoelectron spectroscopy (XPS). The ability of the CaO-SiO2-CuO/PAA specimens to form apatite via a bio-mineralization processwas evaluated by soaking them in simulated body fluid (SBF) in vitro. The surface microstructure and chemical properties after soaking in SBFwere characterized. The release of ions into the SBF was also measured. In addition, rat osteoblasts and two types of bacterial were cultured on the samples to determine their cytocompatibility and antibacterial properties. The results showed that the amorphous CaO-SiO2-CuO materials were successfully decorated into PAA nano-pores and at the same time maintained their nano-featured surfaces. The CaO-SiO2-CuO/PAA samples induced apatite-mineralization in SBF. Meanwhile, the CaO-SiO2-CuO/PAA samples demonstrated great potential for promoting the proliferation of osteoblasts and inhibiting Escherichia coli (E. coli) as well as Staphylococcus. aureus (S. aureus) growth. Specifically, there was an 86.5±4.1% reduction in E. coli, an 88.0 ± 2.2% reduction in S. aureus for the CaO-SiO2-CuO/PAA surfaces compared to PAA controls. The capability to promote osteoblast proliferation and better antibacterial activity of CaO-SiO2- CuO/PAA may be attributed to the fact that Cu ions can be slowly and constantly released from the samples. Importantly, this was achieved without the use of antibiotics or any pharmaceutical agent. Ultimately, these results suggest that the CaO-SiO2-CuO/PAA substrates possessed improved bone cell cytocompatibility and high antibacterial properties leading to a promising bioactive coating candidate for enhanced orthopedic applications.

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具有生物活性的CaO-SiO2-CuO玻璃包埋阳极氧化铝的制备

摘要为了提高多孔阳极氧化铝（PAA）的骨细胞相容性并实现抗菌性能，通过简单的超声辅助溶胶浸渍策略将无定形CaO-SiO2-CuO材料负载到PAA纳米孔（称为CaO-SiO2/CuO/PAA）中。用场发射扫描显微镜（FESEM）、能谱仪（EDS）和X射线光电子能谱仪对所制备的CaO-SiO2-CuO/PAA的表面特征和化学性质进行了研究。通过在体外模拟体液（SBF）中浸泡CaO-SiO2-CuO/PAA样品，评估了它们通过生物矿化过程形成磷灰石的能力。对SBF浸泡后的表面微观结构和化学性能进行了表征。还测量了离子向SBF中的释放。此外，在样品上培养大鼠成骨细胞和两种类型的细菌，以确定它们的细胞相容性和抗菌性能。结果表明，非晶态CaO-SiO2-CuO材料成功地修饰成PAA纳米孔，同时保持了其纳米特征表面。CaO-SiO2-CuO/PAA样品在SBF中诱导磷灰石矿化。同时，CaO-SiO2-CuO/PAA样品在促进成骨细胞增殖、抑制大肠杆菌（E.coli）和葡萄球菌方面表现出巨大的潜力。金黄色葡萄球菌生长。具体而言，与PAA对照相比，CaO-SiO2-CuO/PAA表面的大肠杆菌减少了86.5±4.1%，金黄色葡萄球菌减少了88.0±2.2%。CaO-SiO2-CuO/PAA具有促进成骨细胞增殖的能力和更好的抗菌活性，这可能归因于Cu离子可以从样品中缓慢而持续地释放。重要的是，这是在不使用抗生素或任何药物的情况下实现的。最终，这些结果表明，CaO-SiO2-CuO/PAA基质具有改善的骨细胞细胞相容性和高抗菌性能，为增强骨科应用提供了一种有前途的生物活性涂层候选材料。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical Glasses Materials Science-Surfaces, Coatings and Films

自引率

0.00%

发文量

审稿时长

17 weeks

期刊介绍： Biomedical Glasses is an international Open Access-only journal covering the field of glasses for biomedical applications. The scope of the journal covers the science and technology of glasses and glass-based materials intended for applications in medicine and dentistry. It includes: Chemistry, physics, structure, design and characterization of biomedical glasses Surface science and interactions of biomedical glasses with aqueous and biological media Modeling structure and reactivity of biomedical glasses and their interfaces Biocompatibility of biomedical glasses Processing of biomedical glasses to achieve specific forms and functionality Biomedical glass coatings and composites In vitro and in vivo evaluation of biomedical glasses Glasses and glass-ceramics in engineered regeneration of tissues and organs Glass-based devices for medical and dental applications Application of glasses and glass-ceramics in healthcare.