Effect of Simvastatin Intracerebroventricular Injection (ICV) On Memory and Anxiety in Male Rats in The Presence of Vitamin D Supplementation

Abstract

Introduction: Simvastatin is a lipophilic statin. Lipophilic statins can cross the blood-brain barrier. The present study investigates the effect of simvastatin on working memory and anxiety in adult male rats without brain injury by the ICV method. Method: In this experimental study, 26 male Wistar rats weighing 250-300 g were randomly divided into six groups (n=6) including: control ICV (without injection), Shm (3μl ICV distilled water), simvastatin 28.5 nmol ICV, simvastatin 28.5 nmol ICV + vitamin D 5 µg/kg. Simvastatin treatment was performed for seven days. After the treatment period, the working memory and anxiety was measured. Findings: According to the results, groups receiving simvastatin with a concentration of 28.5 nmol had no significant difference in their memory and anxiety than the control group (P> 0.05). No significant effect on catalase level was observed in any of the groups compared to the control group (P> 0.05). Conclusion: Injection of simvastatin into the brain did not show a significant effect on memory and anxiety in male rats.