K. Samrat, M. Chandraprabha, R. Hari Krishna, R. Sharath, B. Harish
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引用次数: 2
Abstract
Microbial wound infections leading to secondary complications in wound healing has resulted in high demand for therapeutic drugs with improved efficacy. Despite achieving enhanced bio-activity and higher bioavailability compared to its bulk form, nano-sulfur (SNP) has been explored to a very limited extent for wound healing applications. In this work, we prepare biogenic SNP (SNP-B) via simple biogenic technique using pomegranate (Punica granatum) peel extract and demonstrate its antimicrobial and wound healing activity. The SNP-B was characterized using powder x-ray diffractometer, FESEM, transmission electron microscopy and Raman spectroscopy. Different wound models (excision, incision, dead space and burn) were used to assess the wound healing potential of SNP-B. The 2% (w/w) SNP-B treated group exhibited enhanced wound contraction rate (excision wound, 99.62 ± 0.59%; burn wound, 99.46 ± 0.59%), breaking strength (393.2 ± 10.87 g cm−2), and granulation tissue weight (166.8 ± 9.45 mg) compared to the control group (excision wound, 84.24 ± 2.78%; burn wound, 90.58 ± 3.2%; breaking strength, 241.3 ± 16.11 g cm−2; granulation tissue weight, 91.17 ± 7.28 mg). The efficacy of 2% (w/w) SNP-B was comparable to that of standard (5% w/w povidone-iodine ointment) in all the wound models analyzed. The SNP-B showed enhanced antibacterial activity with a MIC value of 90, 80, 80, and 60 μg ml−1 for Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Staphylococcus aureus, respectively. The results obtained prove the potential of SNP-B as a multifunctional therapeutic agent for topical applications.
微生物伤口感染导致伤口愈合中的继发并发症,导致对疗效提高的治疗药物的高需求。尽管与本体形式相比,纳米硫具有更强的生物活性和更高的生物利用度,但其在伤口愈合应用中的探索程度非常有限。在这项工作中,我们使用石榴皮提取物通过简单的生物技术制备了生物SNP(SNP-B),并证明了其抗菌和伤口愈合活性。利用粉末x射线衍射仪、FESEM、透射电子显微镜和拉曼光谱对SNP-B进行了表征。使用不同的伤口模型(切除、切口、死区和烧伤)来评估SNP-B的伤口愈合潜力。2%(w/w)SNP-B治疗组的伤口收缩率(切除伤口,99.62±0.59%;烧伤伤口,99.46±0.59%)、断裂强度(393.2±10.87 g cm−2)、,肉芽组织重量(166.8±9.45 mg)与对照组相比(切除伤口84.24±2.78%;烧伤伤口90.58±3.2%;断裂强度241.3±16.11 g cm−2;肉芽组织质量91.17±7.28 mg)。在所有分析的伤口模型中,2%(w/w)SNP-B的疗效与标准(5%w/w聚维酮碘软膏)相当。SNP-B对铜绿假单胞菌、大肠杆菌、枯草芽孢杆菌和金黄色葡萄球菌的MIC值分别为90、80、80和60μg ml−1,显示出增强的抗菌活性。所获得的结果证明了SNP-B作为局部应用的多功能治疗剂的潜力。
期刊介绍:
Nano Futures mission is to reflect the diverse and multidisciplinary field of nanoscience and nanotechnology that now brings together researchers from across physics, chemistry, biomedicine, materials science, engineering and industry.