Liposome evaluation in inhibiting pump efflux of NorA of Staphylococcus aureus

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemistry and Physics of Lipids Pub Date : 2022-07-01 DOI:10.1016/j.chemphyslip.2022.105204

José Geraldo de Alencar Santos Júnior , Henrique Douglas Melo Coutinho , João Pedro Viana Rodrigues , Vanessa Pinheiro Gonçalves Ferreira , José Bezerra de Araújo Neto , Maria Milene Costa da Silva , Ana Carolina Justino de Araújo , Raimundo Luiz Silva Pereira , Pedro Everson Alexandre de Aquino , Cícera Datiane de Morais Oliveira–Tintino , Roberto Nicolete

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引用次数: 2

Abstract

Liposomes, in addition to providing greater efficacy to antibiotics, decrease toxicity and increase selectivity. This work has as main objectives the sensitization of the need to solve bacterial resistance to antibiotics, addressing the potential of antibiotics carried by liposome. In the preparation of the liposomes, the lipids dipalmitoyl phosphatidylcholine (DPPC), dipalmitoyl phosphatidylserine (DPPS), and cholesterol (COL) with > 99% purity were used. The Staphylococcus aureus strains used were SA-1199B, which expresses the NorA gene encoding the NorA efflux protein, which expels hydrophilic fluoroquinolones and other drugs intercalating DNA dyes, and the wild strain SA-1199. The liposomes associated with antibiotics in the wild type of strain SA-1199 and the carrier strain of pump 1199B, had a better representation of growth inhibition than the wild type strain SA-1199. Given the potential for inhibition of efflux pump seen in the results, we highlight the creation of new drugs or alteration of existing drugs. They are not recognized by the efflux pumps and removed from the target cell.

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脂质体对抑制金黄色葡萄球菌诺拉泵外的评价

脂质体除了对抗生素提供更大的疗效外，还降低了毒性并增加了选择性。这项工作的主要目标是解决细菌对抗生素的耐药性，解决脂质体携带抗生素的潜力。在脂质体的制备中，双棕榈酰磷脂酰胆碱(DPPC)、双棕榈酰磷脂酰丝氨酸(DPPS)和胆固醇(COL)用>纯度为99%。使用的金黄色葡萄球菌菌株为SA-1199B和野生菌株SA-1199，前者表达编码NorA外排蛋白的NorA基因，该蛋白能排出亲水性氟喹诺酮类药物和其他嵌入DNA染料的药物。野生型菌株SA-1199和载菌泵1199B中与抗生素相关的脂质体比野生型菌株SA-1199具有更好的生长抑制作用。鉴于在结果中看到的抑制外排泵的潜力，我们强调新药的创造或现有药物的改变。它们不被外排泵识别并从靶细胞中移除。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemistry and Physics of Lipids 生物-生化与分子生物学

CiteScore

7.60

自引率

2.90%

发文量

审稿时长

40 days

期刊介绍： Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.