Effect of Glucocorticoid Deflazacort on Respiration and Calcium-Dependent Permeability of Rat Liver Mitochondria

IF 1.1 Q4 CELL BIOLOGY

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2021-06-10 DOI:10.1134/S1990747821020033

M. V. Dubinin, A. A. Semenova, E. I. Khoroshavina, K. N. Belosludtsev

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Abstract—

This study examines the effect of glucocorticoid deflazacort on the functioning of rat liver mitochondria. Deflazacort at concentrations up to 100 μM has been shown to have no effect on respiration and oxidative phosphorylation of rat liver mitochondria, energized both in the presence of glutamate/malate (substrates of complex I of the respiratory chain) and succinate (substrate of complex II of the respiratory chain), and also does not affect activity of complexes of the respiratory chain of organelles. It was found that deflazacort does not affect the permeability of the inner membrane of liver mitochondria, but reduces the resistance of organelles to the induction of calcium-dependent MPT pore. In addition, we have found that this glucocorticoid is able to induce a decrease in the level of mitochondrial NAD(P)H, as well as inhibit the production of hydrogen peroxide by organelles. The paper discusses how the effects of deflazacort on mitochondrial function may be related to the therapeutic effects of this agent.

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糖皮质激素对大鼠肝线粒体呼吸及钙依赖性通透性的影响

摘要:本研究探讨糖皮质激素地氮唑柯对大鼠肝脏线粒体功能的影响。浓度高达100 μM的地氮唑柯对大鼠肝脏线粒体的呼吸和氧化磷酸化没有影响，在谷氨酸/苹果酸盐(呼吸链复合体I的底物)和琥珀酸盐(呼吸链复合体II的底物)存在下都能激活，也不影响呼吸链细胞器复合体的活性。结果发现，地拉法柯特不影响肝线粒体内膜的通透性，但降低了细胞器对钙依赖性MPT孔诱导的阻力。此外，我们发现这种糖皮质激素能够诱导线粒体NAD(P)H水平的降低，并抑制细胞器产生过氧化氢。本文讨论了地拉法柯对线粒体功能的影响可能与该药物的治疗效果有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology CELL BIOLOGY-

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.