Human Chorionic Gonadotropin Regulates the Smad Signaling Pathway by Antagonizing TGF-β in Giant Cell Tumor of Bone.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-01-01 DOI:10.2174/1574892818666230413082909

Tangbing Xu, Shenglin Xu, Guangwen Ma, Jun Chang, Chi Zhang, Ping Zhou, Chao Wang, Pengfei Xu, Junjun Yang, Yong Hu, Yunfeng Wu

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Abstract

Background: Giant cell tumor of bone (GCTB) is a locally aggressive bone tumour aggravated by stromal cell proliferation and metastasis.

Objective: We investigated the mechanism of action of human chorionic gonadotropin (HCG) in mediating GCTB proliferation and invasion.

Methods: The expression of HCG was quantified using quantitative real-time PCR. After the primary stromal cells were isolated and identified, the function of HCG in GCTB was estimated using the cell counting kit-8, flow cytometry, scratch experiment, transwell assay, Western blot, and immunofluorescence. Moreover, the mechanism of HCG was assessed through western blotting.

Results: HCG expression was decreased in clinical tissue samples from patients with GCTB. We validated that HCG repressed stromal cell proliferation, migration, invasion, autophagy, and epithelial- mesenchymal transition (EMT) and promoted cell apoptosis in GCTB. We also verified that HCG repressed the autophagy and EMT of stromal cells through the Smad signaling axis in GCTB. HCG inhibited the transduction of the Smad signaling pathway by restraining the binding of the TGF-β II receptor to ligand Activin A.

Conclusion: HCG restrained the Smad signaling pathway by antagonizing TGF-β signaling in GCTB. HCG may serve as a useful patent to treat GCTB.

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人绒毛膜促性腺激素拮抗骨巨细胞瘤TGF-β调控Smad信号通路

骨巨细胞瘤（GCTB）是一种因基质细胞增殖和转移而加重的局部侵袭性骨肿瘤。我们研究了人绒毛膜促性腺激素（HCG）介导GCTB增殖和侵袭的作用机制。HCG的表达使用定量实时PCR进行定量。在分离和鉴定原代基质细胞后，使用细胞计数试剂盒-8、流式细胞术、划痕实验、transwell分析、Western印迹和免疫荧光来评估HCG在GCTB中的功能。此外，通过蛋白质印迹法评估了HCG的作用机制。GCTB患者的临床组织样本中HCG表达降低。我们证实HCG抑制GCTB中基质细胞的增殖、迁移、侵袭、自噬和上皮-间质转化（EMT），并促进细胞凋亡。我们还验证了HCG通过GCTB中的Smad信号轴抑制基质细胞的自噬和EMT。HCG通过抑制TGF-βII受体与配体激活素A的结合来抑制Smad信号通路的转导。HCG可以作为治疗GCTB的有用专利。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.