Temporal transcriptome analysis provides molecular insights into flower development in red-flesh pitaya

IF 2.3 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Electronic Journal of Biotechnology Pub Date : 2022-07-01 DOI:10.1016/j.ejbt.2022.05.005

Zhijiang Wu , Lifang Huang , Fengzhu Huang, Guifeng Lu, Shuotong Wei, Chaoan Liu, Haiyan Deng, Guidong Liang

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引用次数: 4

Abstract

Background

Pitaya is an important economic fruit worldwide due to its numerous health benefits. A systematic understanding of the mechanism underlying flower development is essential to obtain higher fruit yield and quality. However, the genetic mechanism of flower development is not yet investigated in pitaya. Herein, a transcriptome analysis was performed to determine the transcriptional changes during flower development in red-flesh pitaya by utilizing nine different stages of flower development.

Result

A total of 95,412 unigenes were generated with a mean length of 913 nt, and N50 value of 1878 nt. Comparative transcriptomic analysis showed many differentially expressed genes (DEGs) among the flower growth stages. Furthermore, an array of key DEGs were enriched in hormone signaling, transcription, carbohydrate transport, and energy production pathways. In particular, indole-3-acetic acid, abscisic acid, ethylene-responsive transcription factor, constans-like, teosinte branched 1/cycloidea/proliferating cell nuclear antigen factor, apetala1-like, agamous-like MADS-box protein, sepallata, growth-regulating factor, putative axial regulator yabby, leafy/floricaula homolog, and MYB gene-associated transcription factors displayed altered expression, suggesting their critical roles in floral organ development of pitaya. Besides, genes related to sugar synthesis, transportation, and utilization mediate flower growth regulation in pitaya. Eleven genes were selected to perform qRT-PCR analysis to verify the results of the RNA sequencing.

Conclusions

Our results provide important insights into the transcriptional regulation of pitaya flower development. These data resources will be a cornerstone for future research that aims at exploring the genetic control of flower development in pitaya.

How to cite: Wu Z, Huang L, Huang F, et al. Temporal transcriptome analysis provides molecular insights into flower development in red-flesh pitaya. Electron J Biotechnol 2022;58 https://doi.org/10.1016/j.ejbt.2022.05.005

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时间转录组分析提供了红肉火龙果花发育的分子见解

火龙果是一种重要的经济水果，因为它具有许多健康益处。系统地了解花的发育机制对获得更高的果实产量和品质至关重要。然而，火龙果花发育的遗传机制尚未深入研究。本文利用9个不同的花发育阶段，对红肉火龙果进行转录组分析，以确定花发育过程中的转录变化。结果共产生95,412个单基因，平均长度为913 nt, N50值为1878 nt。比较转录组学分析显示，不同花期存在许多差异表达基因(DEGs)。此外，一系列关键的deg在激素信号、转录、碳水化合物运输和能量产生途径中富集。特别是，吲哚-3-乙酸、脱落酸、乙烯应答转录因子、constanslike、teosinte branched 1/cycloidea/增殖细胞核抗原因子、aptala1样、agamus样MADS-box蛋白、sepallata、生长调节因子、轴向调节因子、叶/花椰菜同源物和MYB基因相关转录因子的表达改变，表明它们在火龙果花器官发育中起着关键作用。此外，与糖的合成、运输和利用有关的基因介导火龙果花的生长调控。选择11个基因进行qRT-PCR分析，验证RNA测序结果。结论sour研究结果为火龙果花发育的转录调控提供了重要依据。这些数据资源将为进一步探索火龙果花发育的遗传控制奠定基础。引用方式:吴忠，黄磊，黄峰，等。时间转录组分析提供了红肉火龙果花发育的分子见解。电子J生物技术2022;58 https://doi.org/10.1016/j.ejbt.2022.05.005

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来源期刊

Electronic Journal of Biotechnology 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology. The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th). The following areas are covered in the Journal: • Animal Biotechnology • Biofilms • Bioinformatics • Biomedicine • Biopolicies of International Cooperation • Biosafety • Biotechnology Industry • Biotechnology of Human Disorders • Chemical Engineering • Environmental Biotechnology • Food Biotechnology • Marine Biotechnology • Microbial Biotechnology • Molecular Biology and Genetics •Nanobiotechnology • Omics • Plant Biotechnology • Process Biotechnology • Process Chemistry and Technology • Tissue Engineering